Chapter 1 Zika Virus: A Brief History and Review of Its Pathogenesis Rediscovered

Chapter 2 Zika Virus Isolation, Purification, and Titration

Chapter 3 Evaluating Zika Virus Pathogenesis in Immunocompromised Mice

Chapter 4 Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice

Chapter 5 A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies

Chapter 6 Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test

Chapter 7 Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation

Chapter 8 Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells

Chapter 9 Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA

Chapter 10 Zika Virus Peptide ELISA (ZIKV-NS2B-Concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection

Chapter 11 Zika Virus Amplification Using Strand Displacement Isothermal Method and Sequencing Using Nanopore Technology

Chapter 13 Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus

Chapter 14 Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA

Chapter 15 A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response

Chapter 16 Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes

Chapter 17 Discovery of Zika Virus Dependency and Restriction Factors Using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT)

Chapter 18 Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy

Chapter 19 Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain

Chapter 20 Isolation of Monoclonal Antibodies from Zika Virus-Infected Patient Samples