Chapter title |
The Mitotic Spindle
|
---|---|
Chapter number | 2 |
Book title |
The Mitotic Spindle
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3542-0_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3540-6, 978-1-4939-3542-0
|
Authors |
He, Bin, Cimini, Daniela, Bin He, Daniela Cimini |
Editors |
Paul Chang, Ryoma Ohi |
Abstract |
Mitosis is a highly dynamic process during which the genetic material is equally distributed between two daughter cells. During mitosis, the sister chromatids of replicated chromosomes interact with dynamic microtubules and such interactions lead to stereotypical chromosome movements that eventually result in chromosome segregation and successful cell division. Approaches that allow quantification of microtubule dynamics and chromosome movements are of utmost importance for a mechanistic understanding of mitosis. In this chapter, we describe methods based on activation of photoactivatable green fluorescent protein (PA-GFP) that can be used for quantitative studies of microtubule dynamics and chromosome segregation. |
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