Chapter title |
Isolation and Analysis of Small RNAs from Virus-Infected Plants.
|
---|---|
Chapter number | 12 |
Book title |
Antiviral Resistance in Plants
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Published in |
Methods in molecular biology, June 2012
|
DOI | 10.1007/978-1-61779-882-5_12 |
Pubmed ID | |
Book ISBNs |
978-1-61779-881-8, 978-1-61779-882-5
|
Authors |
Curtin SJ, Zsögön A, Watson JM, Waterhouse PM, Shaun J. Curtin, Agustín Zsögön, John M. Watson, Peter M. Waterhouse, Curtin, Shaun J., Zsögön, Agustín, Watson, John M., Waterhouse, Peter M. |
Abstract |
In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses: Cucumber mosaic virus (CMV), including the CMV Y-Satellite, Turnip mosaic virus (TuMV), Potato leaf roll virus (PLRV), and Tomato spotted wilt virus (TSWV) from inoculated Arabidopsis thaliana plants (Fusaro et al. EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA). |
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