Report for: T Cell Protocols

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Chapter title	T Cell Protocols
Chapter number	9
Book title	T Cell Protocols
Published in	Methods in molecular biology, January 2009
DOI	10.1007/978-1-60327-527-9_9
Pubmed ID	19048217
Book ISBNs	978-1-58829-587-3, 978-1-60327-527-9
Authors	Wilco de Jager, Berent Prakken, Ger T. Rijkers, Jager, Wilco de, Prakken, Berent, Rijkers, Ger T.
Abstract	Subsets of T cells can be distinguished on basis of their cytokine production and secretion profile.With the critical role of cytokines in the regulation of immune and inflammatory responses, cytokines hold the promise to become the ideal biomarkers to monitor development and progression of immune-mediated diseases, study the development of new therapeutic approaches (both in vitro and in vivo) and as outcome parameters. Because of the numerous interactions in the cytokine network, the pleiotropic actions and redundancy, it will be necessary to monitor the complete spectrum of cytokines. As such, the multiplex immunoassay (MIA) is the ideal technique for that purpose. This paper reviews the critical methodological steps of this technique, including the procedures for antibody coupling to beads and matrix effects of biological fluids and buffer solutions. The power and robustness of the MIA technique is illustrated by an analysis of cytokine profiles in juvenile idiopathic arthritis.

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Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Netherlands	1	3%
Canada	1	3%
Unknown	28	93%

Demographic breakdown

Readers by professional status	Count	As %
Student > Ph. D. Student	9	30%
Researcher	5	17%
Student > Master	3	10%
Other	2	7%
Librarian	1	3%
Other	4	13%
Unknown	6	20%

Readers by discipline	Count	As %
Medicine and Dentistry	11	37%
Biochemistry, Genetics and Molecular Biology	3	10%
Immunology and Microbiology	3	10%
Agricultural and Biological Sciences	3	10%
Unspecified	1	3%
Other	2	7%
Unknown	7	23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2012.

All research outputs

#20,159,700

of 22,668,244 outputs

Outputs from Methods in molecular biology

#9,817

of 13,025 outputs

Outputs of similar age

#163,038

of 168,739 outputs

Outputs of similar age from Methods in molecular biology

#149

of 157 outputs

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So far Altmetric has tracked 13,025 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.

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T Cell Protocols

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Mendeley readers

Geographical breakdown

Demographic breakdown

Attention Score in Context