Table of Contents

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   Book Overview
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   Chapter 1 Glasshouse for Maintenance of Virus and Insect Culture
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   Chapter 2 Symptoms of Virus-Infected Plants
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   Chapter 3 Isolation and Diagnosis of Virus Through Indicator Hosts
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   Chapter 4 Host Range of Viruses
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   Chapter 5 Physico-chemical Properties of Virus in Crude Sap
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   Chapter 6 Mechanical Sap Transmission
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   Chapter 7 Transmission Through Grafting and Budding
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   Chapter 8 Transmission Through Dodder
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    Chapter 9 Virus Transmission Through Pollen
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    Chapter 10 Transmission Through Seeds
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    Chapter 11 Transmission of Viruses by Aphids
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    Chapter 12 Transmission of Viruses by Leafhoppers
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    Chapter 13 Transmission of Viruses by Whiteflies
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    Chapter 14 Transmission of Viruses by Thrips
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    Chapter 15 Transmission of Viruses Through Mealybugs
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    Chapter 16 Transmission of Viruses Through Beetles
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    Chapter 17 Transmission of Viruses Through Mites
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    Chapter 18 Transmission of Viruses Through Fungi
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    Chapter 19 Transmission of Viruses Through Nematodes
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    Chapter 20 Storage and Preservation of Plant Virus Cultures
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    Chapter 21 Purification of Plant Viruses
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    Chapter 22 Ultraviolet Absorption Spectra of Purified Virus Preparation
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    Chapter 23 Electron Microscopy and Utramicrotomy
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    Chapter 24 Determination of Coat Protein Molecular Weight of Viruses
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    Chapter 25 Isolation of Nucleic Acid from Purified Virus and Determination of Its Nature
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    Chapter 26 Agarose Gel Electrophoresis for Nucleic Acids
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    Chapter 27 In Vitro Expression of Viral Coat Protein in Prokaryotic System and Its Purification
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    Chapter 28 Production of Polyclonal Antiserum
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    Chapter 29 Production of Monoclonal Antibody
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    Chapter 30 Serological Tests
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    Chapter 31 Isolation of Total DNA from Plants
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    Chapter 32 Isolation of Total RNA from Plants
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    Chapter 33 Isolation of Double-Stranded (ds) RNA from Virus-Infected Plants
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    Chapter 34 Dot-Blot Hybridization Technique
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    Chapter 35 Polymerase Chain Reaction
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    Chapter 36 Real-Time Polymerase Chain Reaction
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    Chapter 37 DNA Microarray for Detection of Plant Viruses
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    Chapter 38 Loop-Mediated Isothermal Amplification (LAMP)
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    Chapter 39 Rolling Circle Amplification (RCA)
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    Chapter 40 Recombinase Polymerase Amplification
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    Chapter 41 Next-Generation Sequencing for Diagnosis of Viruses
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    Chapter 42 Cloning of PCR Product
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    Chapter 43 cDNA Synthesis and Cloning
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    Chapter 44 DNA Sequencing
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    Chapter 45 Sequence Analysis and Phylogenetic Studies
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    Chapter 46 Development of Infectious Clone of Virus
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    Chapter 47 Virus Elimination by Meristem-Tip Culture
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    Chapter 48 Virus Elimination Through Somatic Embryogenesis
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    Chapter 49 Production of Virus-Resistant Plants Through Transgenic Approaches
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    Chapter 50 Production of Virus-Resistant Plants Through CRISPR-Cas Technology