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Functional Genomics

Overview of attention for book
Cover of 'Functional Genomics'

Table of Contents

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    Book Overview
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    Chapter 1 Prediction of Protein Tertiary Structures Using MUFOLD.
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    Chapter 2 Prediction of protein functions.
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    Chapter 3 Genome-wide screens for expressed hypothetical proteins.
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    Chapter 4 Self-Custom-Made SFP Arrays for Nonmodel Organisms.
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    Chapter 5 Construction and analysis of full-length and normalized cDNA libraries from citrus.
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    Chapter 6 Assembling linear DNA templates for in vitro transcription and translation.
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    Chapter 7 Automated Computational Analysis of Genome-Wide DNA Methylation Profiling Data from HELP-Tagging Assays.
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    Chapter 8 Detection of RNA Editing Events in Human Cells Using High-Throughput Sequencing
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    Chapter 9 Comparative study of differential gene expression in closely related bacterial species by comparative hybridization.
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    Chapter 10 Whole-Genome RT-qPCR MicroRNA Expression Profiling
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    Chapter 11 Using Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction to Validate Gene Regulation by PTTG.
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    Chapter 12 FRET-Based Real-Time DNA Microarrays
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    Chapter 13 2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps.
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    Chapter 14 The use of antigen microarrays in antibody profiling.
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    Chapter 15 Limited proteolysis in proteomics using protease-immobilized microreactors.
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    Chapter 16 Mass spectrometry for protein quantification in biomarker discovery.
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    Chapter 17 High-throughput microtitre plate-based assay for DNA topoisomerases.
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    Chapter 18 Microscale Thermophoresis as a Sensitive Method to Quantify Protein: Nucleic Acid Interactions in Solution
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    Chapter 19 Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells.
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    Chapter 20 LuMPIS: Luciferase-Based MBP-Pull-Down Protein Interaction Screening System.
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    Chapter 21 Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins.
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    Chapter 22 Inducible microRNA-Mediated Knockdown of the Endogenous Human Lamin A/C Gene.
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    Chapter 23 Multiple-Gene Silencing Using Antisense RNAs in Escherichia coli.
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    Chapter 24 Functional screen of zebrafish deubiquitylating enzymes by morpholino knockdown and in situ hybridization.
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    Chapter 25 Silencing of gene expression by gymnotic delivery of antisense oligonucleotides.
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    Chapter 26 Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters.
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    Chapter 27 Metabolite Analysis of Cannabis sativa L. by NMR Spectroscopy.
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    Chapter 28 Metabolome Analysis of Gram-Positive Bacteria such as Staphylococcus aureus by GC-MS and LC-MS.
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    Chapter 29 Metabolic Fingerprinting Using Comprehensive Two-Dimensional Gas Chromatography - Time-of-Flight Mass Spectrometry.
Attention for Chapter 5: Construction and analysis of full-length and normalized cDNA libraries from citrus.
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Chapter title
Construction and analysis of full-length and normalized cDNA libraries from citrus.
Chapter number 5
Book title
Functional Genomics
Published in
Methods in molecular biology, November 2011
DOI 10.1007/978-1-61779-424-7_5
Pubmed ID
22130983
Book ISBNs
978-1-61779-423-0, 978-1-61779-424-7
Authors

Marques MC, Perez-Amador MA, M. Carmen Marques, Miguel A. Perez-Amador

Editors

Michael Kaufmann, Claudia Klinger

Abstract

We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of genes.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Student > Doctoral Student 1 33%
Student > Master 1 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 67%
Biochemistry, Genetics and Molecular Biology 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2012.
All research outputs
#20,156,537
of 22,664,644 outputs
Outputs from Methods in molecular biology
#9,804
of 13,025 outputs
Outputs of similar age
#129,839
of 141,616 outputs
Outputs of similar age from Methods in molecular biology
#64
of 76 outputs
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So far Altmetric has tracked 13,025 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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