↓ Skip to main content

Cryopreservation and Freeze-Drying Protocols

Overview of attention for book
Cover of 'Cryopreservation and Freeze-Drying Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Long-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers
  3. Altmetric Badge
    Chapter 2 Cryopreservation and Freeze-Drying Protocols
  4. Altmetric Badge
    Chapter 3 Principles of cryopreservation.
  5. Altmetric Badge
    Chapter 4 Lyophilization of Proteins
  6. Altmetric Badge
    Chapter 5 Vacuum-Drying and Cryopreservation of Prokaryotes
  7. Altmetric Badge
    Chapter 6 Freeze-drying of yeast cultures.
  8. Altmetric Badge
    Chapter 7 Cryopreservation of Yeast Cultures
  9. Altmetric Badge
    Chapter 8 Freeze-Drying Fungi Using a Shelf-Freeze Drier
  10. Altmetric Badge
    Chapter 9 Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying
  11. Altmetric Badge
    Chapter 10 Cryopreservation of Microalgae and Cyanobacteria
  12. Altmetric Badge
    Chapter 11 Cryopreservation of Plant Cell Suspensions
  13. Altmetric Badge
    Chapter 12 Cryopreservation of Shoot Tips and Meristems
  14. Altmetric Badge
    Chapter 13 Cryopreservation of Desiccation-Tolerant Seeds
  15. Altmetric Badge
    Chapter 14 Cryopreservation of Fish Sperm
  16. Altmetric Badge
    Chapter 15 Cryopreservation of Avian Spermatozoa
  17. Altmetric Badge
    Chapter 16 Cryopreservation of Animal and Human Cell Lines
  18. Altmetric Badge
    Chapter 17 Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use
  19. Altmetric Badge
    Chapter 18 Cryopreservation of Human Embryonic Stem Cell Lines
  20. Altmetric Badge
    Chapter 19 Cryopreservation of Primary Animal Cell Cultures
  21. Altmetric Badge
    Chapter 20 Cryopreservation of Red Blood Cells and Platelets
  22. Altmetric Badge
    Chapter 21 Cryopreservation of Mammalian Semen
  23. Altmetric Badge
    Chapter 22 Cryopreservation of Mammalian Oocytes
  24. Altmetric Badge
    Chapter 23 Cryopreservation of Mammalian Embryos
Attention for Chapter 6: Freeze-drying of yeast cultures.
Altmetric Badge

Mentioned by

2 X users


118 Dimensions

Readers on

55 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Freeze-drying of yeast cultures.
Chapter number 6
Book title
Cryopreservation and Freeze-Drying Protocols
Published in
Methods in molecular biology, January 2007
DOI 10.1007/978-1-59745-362-2_6
Pubmed ID
Book ISBNs
978-1-58829-377-0, 978-1-59745-362-2

Bond, Chris, Chris Bond


John G. Day, Glyn N. Stacey


A method is described that allows yeast species to be stored using a variation on the standard freeze-drying method, which employs evaporative cooling in a two-stage process. Yeast cultures are placed in glass ampoules after having been mixed with a lyoprotectant. Primary drying is carried out using a centrifuge head connected to a standard freeze-dryer. Once the centrifuge head is running, air is removed and evaporated liquid is captured in the freeze-dryer. Centrifugation continues for 15 min and primary drying for a further 3 h. The ampoules are constricted using a glass blowing torch. They are then placed on the freeze-dryer manifold for secondary drying under vacuum overnight, using phosphorus pentoxide as a desiccant. The ampoules are sealed and removed from the manifold by melting the constricted section. Although the process causes an initial large drop in viability, further losses after storage are minimal. Yeast strains have remained viable for more than 30 yr when stored using this method and sufficient cells are recovered to produce new working stocks. Although survival rates are strain specific, nearly all National Collection of Yeast Cultures strains covering most yeast genera, have been successfully stored with little or no detectable change in strain characteristics.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 55 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 22%
Student > Bachelor 8 15%
Researcher 6 11%
Student > Ph. D. Student 6 11%
Student > Doctoral Student 3 5%
Other 9 16%
Unknown 11 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 24%
Biochemistry, Genetics and Molecular Biology 12 22%
Chemistry 5 9%
Engineering 4 7%
Immunology and Microbiology 3 5%
Other 7 13%
Unknown 11 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2021.
All research outputs
of 22,830,751 outputs
Outputs from Methods in molecular biology
of 13,126 outputs
Outputs of similar age
of 156,954 outputs
Outputs of similar age from Methods in molecular biology
of 46 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 156,954 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.