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Cryopreservation and Freeze-Drying Protocols

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Cover of 'Cryopreservation and Freeze-Drying Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Long-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers
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    Chapter 2 Cryopreservation and Freeze-Drying Protocols
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    Chapter 3 Principles of cryopreservation.
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    Chapter 4 Lyophilization of Proteins
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    Chapter 5 Vacuum-Drying and Cryopreservation of Prokaryotes
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    Chapter 6 Freeze-drying of yeast cultures.
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    Chapter 7 Cryopreservation of Yeast Cultures
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    Chapter 8 Freeze-Drying Fungi Using a Shelf-Freeze Drier
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    Chapter 9 Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying
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    Chapter 10 Cryopreservation of Microalgae and Cyanobacteria
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    Chapter 11 Cryopreservation of Plant Cell Suspensions
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    Chapter 12 Cryopreservation of Shoot Tips and Meristems
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    Chapter 13 Cryopreservation of Desiccation-Tolerant Seeds
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    Chapter 14 Cryopreservation of Fish Sperm
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    Chapter 15 Cryopreservation of Avian Spermatozoa
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    Chapter 16 Cryopreservation of Animal and Human Cell Lines
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    Chapter 17 Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use
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    Chapter 18 Cryopreservation of Human Embryonic Stem Cell Lines
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    Chapter 19 Cryopreservation of Primary Animal Cell Cultures
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    Chapter 20 Cryopreservation of Red Blood Cells and Platelets
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    Chapter 21 Cryopreservation of Mammalian Semen
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    Chapter 22 Cryopreservation of Mammalian Oocytes
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    Chapter 23 Cryopreservation of Mammalian Embryos
Attention for Chapter 6: Freeze-drying of yeast cultures.
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Chapter title
Freeze-drying of yeast cultures.
Chapter number 6
Book title
Cryopreservation and Freeze-Drying Protocols
Published in
Methods in molecular biology, January 2007
DOI 10.1007/978-1-59745-362-2_6
Pubmed ID
Book ISBNs
978-1-58829-377-0, 978-1-59745-362-2
Authors

Bond, Chris, Chris Bond

Editors

John G. Day, Glyn N. Stacey

Abstract

A method is described that allows yeast species to be stored using a variation on the standard freeze-drying method, which employs evaporative cooling in a two-stage process. Yeast cultures are placed in glass ampoules after having been mixed with a lyoprotectant. Primary drying is carried out using a centrifuge head connected to a standard freeze-dryer. Once the centrifuge head is running, air is removed and evaporated liquid is captured in the freeze-dryer. Centrifugation continues for 15 min and primary drying for a further 3 h. The ampoules are constricted using a glass blowing torch. They are then placed on the freeze-dryer manifold for secondary drying under vacuum overnight, using phosphorus pentoxide as a desiccant. The ampoules are sealed and removed from the manifold by melting the constricted section. Although the process causes an initial large drop in viability, further losses after storage are minimal. Yeast strains have remained viable for more than 30 yr when stored using this method and sufficient cells are recovered to produce new working stocks. Although survival rates are strain specific, nearly all National Collection of Yeast Cultures strains covering most yeast genera, have been successfully stored with little or no detectable change in strain characteristics.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 55 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 22%
Student > Bachelor 8 15%
Researcher 6 11%
Student > Ph. D. Student 6 11%
Student > Doctoral Student 3 5%
Other 9 16%
Unknown 11 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 24%
Biochemistry, Genetics and Molecular Biology 12 22%
Chemistry 5 9%
Engineering 4 7%
Immunology and Microbiology 3 5%
Other 7 13%
Unknown 11 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2021.
All research outputs
#14,239,950
of 22,830,751 outputs
Outputs from Methods in molecular biology
#4,191
of 13,126 outputs
Outputs of similar age
#131,715
of 156,954 outputs
Outputs of similar age from Methods in molecular biology
#32
of 46 outputs
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So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
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