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Lipases and Phospholipases

Overview of attention for book
Cover of 'Lipases and Phospholipases'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Lipases: An Overview
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    Chapter 2 Lipases or Esterases: Does It Really Matter? Toward a New Bio-Physico-Chemical Classification
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    Chapter 3 Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus
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    Chapter 4 Phospholipases: An Overview
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    Chapter 5 High-Throughput Screening Method for Lipases/Esterases
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    Chapter 6 Functional-based screening methods for lipases, esterases, and phospholipases in metagenomic libraries.
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    Chapter 7 Plant Lipases: Partial Purification of Carica papaya Lipase
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    Chapter 8 Plant Phospholipases: An Overview
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    Chapter 9 Lipase and Phospholipase Inhibitors: Design and Applications
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    Chapter 10 High-Throughput Assay of Secreted Phospholipases A 2 Inhibitors
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    Chapter 11 Heterologous Expression Systems for Lipases: A Review
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    Chapter 12 Streptomyces Phospholipase D Cloning and Production
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    Chapter 13 Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses.
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    Chapter 14 Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite
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    Chapter 15 Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27.
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    Chapter 16 Purification, Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase
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    Chapter 17 Phospholipase A2 Purification and Characterization: A Case Study
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    Chapter 18 The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Enzymes
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    Chapter 19 Molecular Modeling of Lipase Binding to a Substrate–Water Interface
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    Chapter 20 Different Strategies for Hyperactivation of Lipase Biocatalysts
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    Chapter 21 Rational Design of Immobilized Lipases and Phospholipases
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    Chapter 22 Thermodynamical Methods for the Optimization of Lipase-Catalyzed Reactions
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    Chapter 23 Lipases as Biocatalysts for the Synthesis of Structured Lipids
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    Chapter 24 Lipase-Catalyzed Modification of Phenolic Antioxidants
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    Chapter 25 Lipases as Catalysts in Synthesis of Fine Chemicals
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    Chapter 26 Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides
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    Chapter 27 Lipases as biocatalyst for biodiesel production.
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    Chapter 28 Synthesis of Biodegradable Polymers Using Biocatalysis with Yarrowia lipolytica Lipase
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    Chapter 29 Phospholipases in Food Industry: A Review
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    Chapter 30 Lipase and Phospholipase Biosensors: A Review
Attention for Chapter 13: Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses.
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Chapter title
Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses.
Chapter number 13
Book title
Lipases and Phospholipases
Published in
Methods in molecular biology, March 2012
DOI 10.1007/978-1-61779-600-5_13
Pubmed ID
22426721
Book ISBNs
978-1-61779-599-2, 978-1-61779-600-5
Authors

Gomez-Cambronero J, Henkels KM, Julian Gomez-Cambronero, Karen M. Henkels, Gomez-Cambronero, Julian, Henkels, Karen M.

Abstract

The enzyme PLD hydrolyzes phosphodiester bonds of lipids in cell membranes. Phosphatidic acid, a chief product of PLD enzymatic activity, is a pleiotropic second messenger with key roles in membrane trafficking, cell invasion, cell growth, and anti-apoptosis. We describe in the present study molecular, cellular, and physiological methods to understand the mechanism of how the PLD2 isozyme regulates the process of inflammation. We describe here (1) a method that details phospholipase D2 (PLD2) cloning in the pBac expression vector, (2) the large-scale infection of Sf21 insect cells for protein production, (3) protein purification by TALON cobalt metal affinity matrix and subsequent assessment of PLD2 protein and lipase activity, (4) application of purified PLD2 protein for the study of Rac2 GTPase biology involving GTP binding by a pull-down assay and GTP/GDP exchange activity, (5) a method of PLD2 expression that involves mammalian cells, (6) a physiological application as relates to adhesion, chemotaxis, and phagocytosis, and (7) a model that integrates the results of a PLD-GTPase interaction from the molecular to the physiological contexts.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 20%
Unknown 4 80%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 60%
Student > Bachelor 1 20%
Student > Doctoral Student 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 60%
Biochemistry, Genetics and Molecular Biology 1 20%
Unknown 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2012.
All research outputs
#18,305,470
of 22,663,969 outputs
Outputs from Methods in molecular biology
#7,813
of 13,024 outputs
Outputs of similar age
#123,613
of 159,946 outputs
Outputs of similar age from Methods in molecular biology
#39
of 70 outputs
Altmetric has tracked 22,663,969 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,024 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 70 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.

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