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High-Throughput Protein Production and Purification

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Cover of 'High-Throughput Protein Production and Purification'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of High-Throughput Cloning Methods for the Post-genomic Era
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    Chapter 2 Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins
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    Chapter 3 Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins
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    Chapter 4 High-Throughput Protein Production in Yeast
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    Chapter 5 A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells
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    Chapter 6 A High-Throughput Automated Protein Folding System
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    Chapter 7 High-Throughput Production of Oxidized Animal Toxins in Escherichia coli
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    Chapter 8 High-Throughput Purification of Protein Kinases from Escherichia coli and Insect Cells
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    Chapter 9 Parallelized Microscale Expression of Soluble scFv
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    Chapter 10 High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression
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    Chapter 11 High-Throughput Protein Production of Membrane Proteins in Saccharomyces cerevisiae
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    Chapter 12 High-Throughput E. coli Cell-Free Expression: From PCR Product Design to Functional Validation of GPCR
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    Chapter 13 High-Throughput Site-Directed Mutagenesis
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    Chapter 14 Hot CoFi Blot: A High-Throughput Colony-Based Screen for Identifying More Thermally Stable Protein Variants
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    Chapter 15 High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System
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    Chapter 16 High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization
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    Chapter 17 High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography
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    Chapter 18 A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production
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    Chapter 19 Adaption of the Leishmania Cell-Free Expression System to High-Throughput Analysis of Protein Interactions
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    Chapter 20 High-Throughput Protein–Protein Interaction Assays Using Tripartite Split-GFP Complementation
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    Chapter 21 High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay
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    Chapter 22 High-Throughput Protein Analysis Using Negative Stain Electron Microscopy and 2D Classification
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    Chapter 23 High-Throughput Protein Production Combined with High- Throughput SELEX Identifies an Extensive Atlas of Ciona robusta Transcription Factor DNA-Binding Specificities
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    Chapter 24 High-Throughput Micro-Characterization of RNA–Protein Interactions
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