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High-Throughput Protein Production and Purification

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Cover of 'High-Throughput Protein Production and Purification'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of High-Throughput Cloning Methods for the Post-genomic Era
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    Chapter 2 Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins
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    Chapter 3 Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins
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    Chapter 4 High-Throughput Protein Production in Yeast
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    Chapter 5 A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells
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    Chapter 6 A High-Throughput Automated Protein Folding System
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    Chapter 7 High-Throughput Production of Oxidized Animal Toxins in Escherichia coli
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    Chapter 8 High-Throughput Purification of Protein Kinases from Escherichia coli and Insect Cells
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    Chapter 9 Parallelized Microscale Expression of Soluble scFv
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    Chapter 10 High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression
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    Chapter 11 High-Throughput Protein Production of Membrane Proteins in Saccharomyces cerevisiae
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    Chapter 12 High-Throughput E. coli Cell-Free Expression: From PCR Product Design to Functional Validation of GPCR
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    Chapter 13 High-Throughput Site-Directed Mutagenesis
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    Chapter 14 Hot CoFi Blot: A High-Throughput Colony-Based Screen for Identifying More Thermally Stable Protein Variants
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    Chapter 15 High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System
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    Chapter 16 High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization
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    Chapter 17 High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography
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    Chapter 18 A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production
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    Chapter 19 Adaption of the Leishmania Cell-Free Expression System to High-Throughput Analysis of Protein Interactions
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    Chapter 20 High-Throughput Protein–Protein Interaction Assays Using Tripartite Split-GFP Complementation
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    Chapter 21 High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay
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    Chapter 22 High-Throughput Protein Analysis Using Negative Stain Electron Microscopy and 2D Classification
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    Chapter 23 High-Throughput Protein Production Combined with High- Throughput SELEX Identifies an Extensive Atlas of Ciona robusta Transcription Factor DNA-Binding Specificities
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    Chapter 24 High-Throughput Micro-Characterization of RNA–Protein Interactions
Attention for Chapter 14: Hot CoFi Blot: A High-Throughput Colony-Based Screen for Identifying More Thermally Stable Protein Variants
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Chapter title
Hot CoFi Blot: A High-Throughput Colony-Based Screen for Identifying More Thermally Stable Protein Variants
Chapter number 14
Book title
High-Throughput Protein Production and Purification
Published by
Humana, New York, NY, July 2019
DOI 10.1007/978-1-4939-9624-7_14
Pubmed ID
Book ISBNs
978-1-4939-9623-0, 978-1-4939-9624-7
Authors

Ignacio Asial, Pär Nordlund, Sue-Li Dahlroth, Asial, Ignacio, Nordlund, Pär, Dahlroth, Sue-Li

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Professor > Associate Professor 1 10%
Student > Ph. D. Student 1 10%
Student > Postgraduate 1 10%
Student > Master 1 10%
Other 0 0%
Unknown 3 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 40%
Agricultural and Biological Sciences 1 10%
Neuroscience 1 10%
Unknown 4 40%