Chapter title |
Analysis of Electroblotted Proteins by Mass Spectrometry.
|
---|---|
Chapter number | 25 |
Book title |
Detection of Blotted Proteins
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2718-0_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2717-3, 978-1-4939-2718-0
|
Authors |
Luque-Garcia, Jose L, Neubert, Thomas A, Jose L. Luque-Garcia, Thomas A. Neubert, Luque-Garcia, Jose L., Neubert, Thomas A. |
Abstract |
Identification of proteins by mass spectrometry is crucial for better understanding of many biological, biochemical, and biomedical processes. Here we describe two methods for the identification of electroblotted proteins by on-membrane digestion prior to analysis by mass spectrometry. These on-membrane methods take approximately half the time of in-gel digestion and provide better digestion efficiency, due to the better accessibility of the protease to the proteins adsorbed onto the nitrocellulose, and better protein sequence coverage, especially for membrane proteins where large and hydrophobic peptides are commonly present. |
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