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Mucins

Overview of attention for book
Cover of 'Mucins'

Table of Contents

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    Book Overview
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    Chapter 1 Mucin methods: genes encoding mucins and their genetic variation with a focus on gel-forming mucins.
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    Chapter 2 Gel-forming and cell-associated mucins: preparation for structural and functional studies.
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    Chapter 3 Detecting, visualising, and quantifying mucins.
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    Chapter 4 Mass Spectrometric Analysis of Mucin Core Proteins
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    Chapter 5 O-glycoprotein biosynthesis: site localization by edman degradation and site prediction based on random Peptide substrates.
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    Chapter 6 Analysis of Assembly of Secreted Mucins
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    Chapter 7 MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling, and Release Through Exosomes
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    Chapter 8 Glycomic Work-Flow for Analysis of Mucin O-Linked Oligosaccharides
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    Chapter 9 O-Glycomics: Profi ling and Structural Analysis of Mucin-type O-linked Glycans
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    Chapter 10 O-Glycoproteomics: Site-Specifi c O-Glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry
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    Chapter 11 Mucins
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    Chapter 12 Assessment of Mucus Thickness and Production In Situ
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    Chapter 13 Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH
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    Chapter 14 Ex Vivo Measurements of Mucus Secretion by Colon Explants
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    Chapter 15 Establishment of Respiratory Air–Liquid Interface Cultures and Their Use in Studying Mucin Production, Secretion, and Function
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    Chapter 16 Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures
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    Chapter 17 Assessment of intracellular mucin content in vivo.
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    Chapter 18 Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo
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    Chapter 19 Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro
Attention for Chapter 17: Assessment of intracellular mucin content in vivo.
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Chapter title
Assessment of intracellular mucin content in vivo.
Chapter number 17
Book title
Mucins
Published in
Methods in molecular biology, December 2011
DOI 10.1007/978-1-61779-513-8_17
Pubmed ID
22259143
Book ISBNs
978-1-61779-512-1, 978-1-61779-513-8
Authors

Piccotti L, Dickey BF, Evans CM, Piccotti, Lucia, Dickey, Burton F., Evans, Christopher M., Lucia Piccotti, Burton F. Dickey, Christopher M. Evans

Abstract

Airway mucus presents a first line of defense against inhaled materials. It also, however, is a significant pathological contributor to chronic lung diseases, such as asthma, cystic fibrosis, and chronic obstructive pulmonary disease. Thus, gaining a better understanding of the mechanisms of mucus production and secretion is an important goal for improving respiratory health. Mucins, the chief glycoprotein components of airway mucus, are very large polymeric glycoproteins, and measuring their production and secretion in experimental animals presents significant technical challenges. Over the past several years, we have developed assays for accurately quantifying mucin production and secretion using histological and biochemical assays. These methods are described here.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 7%
Unknown 14 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Student > Doctoral Student 3 20%
Student > Bachelor 3 20%
Researcher 2 13%
Other 1 7%
Other 1 7%
Unknown 1 7%
Readers by discipline Count As %
Medicine and Dentistry 4 27%
Biochemistry, Genetics and Molecular Biology 3 20%
Agricultural and Biological Sciences 2 13%
Nursing and Health Professions 1 7%
Immunology and Microbiology 1 7%
Other 3 20%
Unknown 1 7%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2012.
All research outputs
#18,304,230
of 22,662,201 outputs
Outputs from Methods in molecular biology
#7,811
of 13,021 outputs
Outputs of similar age
#195,306
of 241,497 outputs
Outputs of similar age from Methods in molecular biology
#313
of 461 outputs
Altmetric has tracked 22,662,201 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,021 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 241,497 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 461 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.

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