| Chapter title |
Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells.
|
|---|---|
| Chapter number | 19 |
| Book title |
Gene Therapy of Cancer
|
| Published in |
Methods in molecular biology, February 2009
|
| DOI | 10.1007/978-1-59745-561-9_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-934115-85-5, 978-1-59745-561-9
|
| Authors |
Devi, Gayathri R, Devi, Gayathri R., Gayathri R. Devi |
| Abstract |
Phosphorodiamidate morpholino oligomers (PMO), which have a neutral chemistry, are extensively being used as tools for selective inhibition of gene expression in cell culture models and are currently in human clinical trials. PMO oligomers possess a unique structure, in which the deoxyribose moiety of DNA is replaced with a six-membered morpholine ring and the charged phosphodiester internucleoside linkages are replaced with neutral phosphorodiamidate linkages. PMO internalization in uptake-permissive cells has been observed to be specific, saturable, and energy-dependent, suggesting a receptor-mediated uptake mechanism. Understanding PMO transport should facilitate the design of more effective synthetic antisense oligomers as therapeutic agents. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 17% |
| India | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Unknown | 2 | 33% |