Chapter title |
Recombinant Gene Expression
|
---|---|
Chapter number | 26 |
Book title |
Recombinant Gene Expression
|
Published in |
Methods in molecular biology, November 2011
|
DOI | 10.1007/978-1-61779-433-9_26 |
Pubmed ID | |
Book ISBNs |
978-1-61779-432-2, 978-1-61779-433-9
|
Authors |
Jianfeng Xu, Marcia J. Kieliszewski |
Editors |
Argelia Lorence |
Abstract |
Plant cell suspension culture integrates the merits of whole-plant systems with those of microbial fermentation and mammalian cell culture, and has been recognized as a promising alternative biosynthetic platform for valuable proteins. However, the low protein productivity dilemma has been the bottleneck toward commercializing this technology. Here, we describe a new technology, termed hydroxyproline (Hyp)-Glyco technology, that dramatically increases the yield of secreted recombinant proteins from cultured plant cells by expressing them as fusions with a novel glycomodule tag comprising an Hyp-rich repetitive peptide (HypRP) backbone that is subsequently glycosylated through the Hyp residues. The extensive glycosylation of the HypRP tags greatly extends the serum half-life of small therapeutic proteins, such as interferon α2b or human growth hormone, without significantly impairing their bioactivities and the tag greatly enhances solubility. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 5% |
Unknown | 18 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 32% |
Student > Master | 4 | 21% |
Researcher | 4 | 21% |
Other | 2 | 11% |
Professor > Associate Professor | 2 | 11% |
Other | 0 | 0% |
Unknown | 1 | 5% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 53% |
Biochemistry, Genetics and Molecular Biology | 7 | 37% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Unknown | 1 | 5% |