Chapter title |
Detection of Trypanosoma cruzi by Polymerase Chain Reaction
|
---|---|
Chapter number | 12 |
Book title |
Clinical Applications of PCR
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3360-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3358-7, 978-1-4939-3360-0
|
Authors |
Márquez, María Elizabeth, Concepción, Juan Luis, González-Marcano, Eglys, Mondolfi, Alberto Paniz, María Elizabeth Márquez, Juan Luis Concepción, Eglys González-Marcano, Alberto Paniz Mondolfi |
Editors |
Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel |
Abstract |
American Trypanosomiasis (Chagas disease) is an infectious disease caused by the hemoflagellate parasite Trypanosoma cruzi which is transmitted by reduviid bugs. T. cruzi infection occurs in a broad spectrum of reservoir animals throughout North, Central, and South America and usually evolves into an asymptomatic chronic clinical stage of the disease in which diagnosis is often challenging. This chapter describes the application of polymerase chain reaction (PCR) for the detection of Trypanosoma cruzi DNA including protocols for sample preparation, DNA extraction, and target amplification methods. |
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