Chapter title |
Methods for Tumor Targeting with Salmonella typhimurium A1-R.
|
---|---|
Chapter number | 13 |
Book title |
Bacterial Therapy of Cancer
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3515-4_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3513-0, 978-1-4939-3515-4
|
Authors |
Hoffman, Robert M, Zhao, Ming, Robert M. Hoffman, Ming Zhao |
Abstract |
Salmonella typhimurium A1-R (S. typhimurium A1-R) has shown great preclinical promise as a broad-based anti-cancer therapeutic (please see Chapter 1 ). The present chapter describes materials and methods for the preclinical study of S. typhimurium A1-R in clinically-relevant mouse models. Establishment of orthotopic metastatic mouse models of the major cancer types is described, as well as other useful models, for efficacy studies of S. typhimurium A1-R or other tumor-targeting bacteria, as well. Imaging methods are described to visualize GFP-labeled S. typhimurium A1-R, as well as GFP- and/or RFP-labeled cancer cells in vitro and in vivo, which S. typhimurium A1-R targets. The mouse models include metastasis to major organs that are life-threatening to cancer patients including the liver, lung, bone, and brain and how to target these metastases with S. typhimurium A1-R. Various routes of administration of S. typhimurium A1-R are described with the advantages and disadvantages of each. Basic experiments to determine toxic effects of S. typhimurium A1-R are also described. Also described are methodologies for combining S. typhimurium A1-R and chemotherapy. The testing of S. typhimurium A1-R on patient tumors in patient-derived orthotopic xenograft (PDOX) mouse models is also described. The major methodologies described in this chapter should be translatable for clinical studies. |
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Mendeley readers
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Researcher | 5 | 21% |
Student > Bachelor | 3 | 13% |
Student > Ph. D. Student | 2 | 8% |
Unspecified | 1 | 4% |
Other | 2 | 8% |
Unknown | 6 | 25% |
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Agricultural and Biological Sciences | 2 | 8% |
Other | 5 | 21% |
Unknown | 7 | 29% |