Chapter title |
MicroRNA (miRNA) Profiling.
|
---|---|
Chapter number | 8 |
Book title |
Cancer Gene Profiling
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3204-7_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3203-0, 978-1-4939-3204-7
|
Authors |
Gao, Lu, Jiang, Feng, Lu Gao, Feng Jiang |
Editors |
Robert Grützmann, Christian Pilarsky |
Abstract |
MicroRNAs (miRNAs) are small, highly conserved noncoding RNA molecules involved in the regulation of gene expression. Since each miRNA regulates the expression of hundreds of target mRNAs, miRNAs could function as master coordinators, efficiently regulating fundamental cellular processes, including proliferation, apoptosis, and development. Furthermore, miRNAs may provide useful diagnostic and therapeutic targets in a variety of diseases. However, miRNA expression profiling is essential for the investigation of the biological functions and clinical applications of miRNAs. Therefore, in this chapter, we review and discuss commonly used techniques for miRNAs profiling, as well as their advantages and restrictions. |
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