| Chapter title |
In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells.
|
|---|---|
| Chapter number | 15 |
| Book title |
Platelets and Megakaryocytes
|
| Published in |
Methods in molecular biology, January 2012
|
| DOI | 10.1007/978-1-61779-307-3_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-306-6, 978-1-61779-307-3
|
| Authors |
Naoya Takayama, Koji Eto, Takayama, Naoya, Eto, Koji |
| Abstract |
Human embryonic stem cells (hESCs) represent a potential source of blood cells for transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. Moreover, human-induced pluripotent stem cells (hiPSCs), recently established by defined reprogramming factors expressed in somatic cells, represent a further source for the generation of hematopoietic cells. When undifferentiated hESCs or hiPSCs are cultured on either mesenchymal C3H10T1/2 cells or OP-9 stromal cells, they can be differentiated into a hematopoietic niche that concentrates hematopoietic progenitors, which we named "embryonic stem cell-derived sacs" (ES-sacs). We have optimized the in vitro culture condition for obtaining mature megakaryocytes derived from the hematopoietic progenitors within ES-sacs, which are then able to release platelets. These in vitro-generated platelets display integrin activation capability, indicating normal hemostatic function. This novel protocol thus provides a means of generating platelets from hESCs as well as hiPSCs, for the study of normal human thrombopoiesis and also thrombopoiesis in disease conditions using patient-specific hiPSCs. |
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Demographic breakdown
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Mendeley readers
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| United Kingdom | 1 | 4% |
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|---|---|---|
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| Student > Ph. D. Student | 4 | 16% |
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| Student > Postgraduate | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 5 | 20% |
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| Computer Science | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 5 | 20% |