Chapter title |
Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe.
|
---|---|
Chapter number | 17 |
Book title |
Plant Cell Division
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3142-2_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3141-5, 978-1-4939-3142-2
|
Authors |
Gerbeau-Pissot, Patricia, Der, Christophe, Grebe, Markus, Stanislas, Thomas, Patricia Gerbeau-Pissot, Christophe Der, Markus Grebe, Thomas Stanislas |
Abstract |
Eukaryotic cells contain membranes exhibiting different levels of lipid order mostly related to their relative amount of sterol-rich domains, thought to mediate temporal and spatial organization of cellular processes. We previously provided evidence in Arabidopsis thaliana that sterols are crucial for execution of cytokinesis, the last stage of cell division. Recently, we used di-4-ANEPPDHQ, a fluorescent probe sensitive to order of lipid phases, to quantify the level of membrane order of the cell plate, the membrane structure separating daughter cells during somatic cytokinesis of higher plant cells. By employing quantitative, ratiometric fluorescence microscopy for mapping localized lipid order levels, we revealed that the Arabidopsis cell plate represents a high-lipid-order domain of the plasma membrane. Here, we describe step-by-step protocols and troubleshooting for ratiometric live imaging procedures employing the di-4-ANEPPDHQ fluorescent probe for quantification of membrane lipid order during plant cell division in suspension cell cultures and roots of Arabidopsis thaliana. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 23% |
Professor > Associate Professor | 2 | 15% |
Student > Master | 2 | 15% |
Unspecified | 1 | 8% |
Student > Bachelor | 1 | 8% |
Other | 2 | 15% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 38% |
Biochemistry, Genetics and Molecular Biology | 4 | 31% |
Arts and Humanities | 1 | 8% |
Unspecified | 1 | 8% |
Unknown | 2 | 15% |