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Recombinant Protein Expression in Mammalian Cells

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Cover of 'Recombinant Protein Expression in Mammalian Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Transient Gene Expression in Suspension HEK293-EBNA1 Cells
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    Chapter 2 Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells
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    Chapter 3 PEI-Mediated Transient Gene Expression in CHO Cells
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    Chapter 4 Stable Expression by Lentiviral Transduction of Cells
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    Chapter 5 Inducible Protein Production in 293 Cells Using the piggyBac Transposon System
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    Chapter 6 Recombinant CHO Cell Pool Generation Using piggyBac Transposon System
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    Chapter 7 Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression
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    Chapter 8 Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
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    Chapter 9 CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors
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    Chapter 10 Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
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    Chapter 11 Continuous and Integrated Expression and Purification of Recombinant Antibodies
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    Chapter 12 High Throughput Transfection of HEK293 Cells for Transient Protein Production
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    Chapter 13 Microfluidic Transfection for High-Throughput Mammalian Protein Expression
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    Chapter 14 Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production
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    Chapter 15 Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells
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    Chapter 16 Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells
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    Chapter 17 Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)
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    Chapter 18 Considerations in the Use of Codon Optimization for Recombinant Protein Expression
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    Chapter 19 Versatile Cell-Free Protein Synthesis Systems Based on Chinese Hamster Ovary Cells
Attention for Chapter 1: Transient Gene Expression in Suspension HEK293-EBNA1 Cells
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