Chapter title |
Detection of Circulating Tumor DNA in the Blood of Cancer Patients: An Important Tool in Cancer Chemoprevention.
|
---|---|
Chapter number | 5 |
Book title |
Cancer Chemoprevention
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3191-0_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3190-3, 978-1-4939-3191-0
|
Authors |
Ulz, Peter, Auer, Martina, Heitzer, Ellen, Peter Ulz, Martina Auer, Ellen Heitzer |
Abstract |
Liquid biopsies represent novel promising tools to determine the impact of clonal heterogeneity on clinical outcomes with the potential to identify novel therapeutic targets in cancer patients. We developed a low-coverage whole-genome sequencing approach in order to noninvasively establish copy number aberrations in plasma DNA from metastasized cancer patients. Using plasma-Seq we were able to monitor genetic evolution including the acquirement of novel copy number changes, such as focal amplifications and chromosomal polysomies. The big advantage of our approach is that it can be performed on a benchtop sequencer, speed, and cost-effectiveness. Therefore, plasma-Seq represents an easy, fast, and affordable tool to provide the urgently needed genetic follow-up data. Here we describe our method including plasma DNA extraction, library preparation, and bioinformatic analyses. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 6% |
Ireland | 1 | 3% |
Unknown | 30 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 30% |
Researcher | 5 | 15% |
Student > Postgraduate | 4 | 12% |
Student > Master | 4 | 12% |
Student > Bachelor | 3 | 9% |
Other | 3 | 9% |
Unknown | 4 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 27% |
Medicine and Dentistry | 8 | 24% |
Agricultural and Biological Sciences | 4 | 12% |
Engineering | 3 | 9% |
Sports and Recreations | 1 | 3% |
Other | 3 | 9% |
Unknown | 5 | 15% |