Chapter title |
In Vivo Analysis of Alpha-1-Antitrypsin Functions in Autoimmune Disease Models
|
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Chapter number | 10 |
Book title |
Serpins
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Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8645-3_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8644-6, 978-1-4939-8645-3
|
Authors |
Sihong Song, Yuanqing Lu, Ahmed S. Elshikha, Song, Sihong, Lu, Yuanqing, Elshikha, Ahmed S. |
Abstract |
Alpha-1-antitrypsin (AAT) is a circulating protein, a serpin, with multiple protective functions. Beside the well-known proteinase inhibitory function, which protects the lungs from chronic obstructive pulmonary disease (COPD), many studies have shown that AAT inhibits pro-inflammatory cytokine gene expression and functions. These anti-inflammatory and immune-regulatory properties have led to studies testing the therapeutic effect of AAT in autoimmune disease models. Initially, a study using recombinant adeno-associated viral (rAAV) vector showed that AAT gene therapy prevented type 1 diabetes (T1D) development in a nonobese diabetic (NOD) mouse model. Consequently, several studies confirmed that AAT therapy prevented and reversed T1D. AAT therapy has also been tested and has demonstrated protective effects in a collagen-induced arthritis model and a systemic lupus erythematosus (SLE) mouse model. This chapter describes methods that evaluate AAT functions in autoimmune mouse models. |
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