| Chapter title |
Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment
|
|---|---|
| Chapter number | 187 |
| Book title |
Stem Cell Niche
|
| Published in |
Methods in molecular biology, August 2018
|
| DOI | 10.1007/7651_2018_187 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-9507-3, 978-1-4939-9508-0
|
| Authors |
Madeline Campbell, Mamta Chabria, Gemma A. Figtree, Liudmila Polonchuk, Carmine Gentile |
| Abstract |
Our laboratory has recently developed a novel three-dimensional in vitro model of the human heart, which we call the vascularized cardiac spheroid (VCS). These better recapitulate the human heart's cellular and extracellular microenvironment compared to the existing in vitro models. To achieve this, human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes, cardiac fibroblasts, and human coronary artery endothelial cells are co-cultured in hanging drop culture in ratios similar to those found in the human heart in vivo. The resulting three-dimensional cellular organization, extracellular matrix, and microvascular network formation throughout the VCS has been shown to mimic the one present in the human heart tissue. Therefore, VCSs offer a promising platform to study cardiac physiology, disease, and pharmacology, as well as bioengineering constructs to regenerate heart tissue. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 51 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 20% |
| Student > Master | 9 | 18% |
| Student > Bachelor | 7 | 14% |
| Student > Doctoral Student | 3 | 6% |
| Researcher | 3 | 6% |
| Other | 4 | 8% |
| Unknown | 15 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 33% |
| Engineering | 6 | 12% |
| Medicine and Dentistry | 5 | 10% |
| Agricultural and Biological Sciences | 3 | 6% |
| Chemical Engineering | 1 | 2% |
| Other | 5 | 10% |
| Unknown | 14 | 27% |