Chapter title |
Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology
|
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Chapter number | 4 |
Book title |
Xenopus
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8784-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8783-2, 978-1-4939-8784-9
|
Authors |
Tom Van Nieuwenhuysen, Kris Vleminckx, Nieuwenhuysen, Tom Van, Vleminckx, Kris |
Abstract |
Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward transcription activator-like effector nucleases (TALENs), adapted from plant pathogenic Xanthomonas species. Although functional genomics are more recently dominated by the even faster and more convenient CRISPR/Cas9 technology, the use of TALENs may still be preferred in a number of cases. We have successfully implemented this technology in Xenopus and in this chapter we describe our working protocol for targeted genome editing in X. tropicalis using TALENs. |
X Demographics
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 7 | 100% |
Demographic breakdown
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Student > Master | 2 | 29% |
Researcher | 2 | 29% |
Professor | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Unknown | 1 | 14% |
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Biochemistry, Genetics and Molecular Biology | 3 | 43% |
Agricultural and Biological Sciences | 1 | 14% |
Unknown | 3 | 43% |