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Mendeley readers
Chapter title |
In-Gel Protein Phosphatase Assay Using Fluorogenic Substrates
|
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Chapter number | 20 |
Book title |
Protein Gel Detection and Imaging
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Published in |
Methods in molecular biology, August 2018
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DOI | 10.1007/978-1-4939-8745-0_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8744-3, 978-1-4939-8745-0
|
Authors |
Isamu Kameshita, Noriyuki Sueyoshi, Atsuhiko Ishida, Kameshita, Isamu, Sueyoshi, Noriyuki, Ishida, Atsuhiko |
Abstract |
Protein phosphorylation plays important roles in regulating a variety of biological processes in animals, plants, and microorganisms. Therefore, it is important to use appropriate techniques to detect and analyze protein kinases and protein phosphatases. In this chapter, we describe the method to detect protein phosphatase activities using fluorogenic substrates such as 4-methylumbelliferyl phosphate (MUP) after separating proteins by one-dimensional or two-dimensional polyacrylamide gel electrophoresis. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 40% |
Researcher | 2 | 40% |
Student > Master | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 80% |
Agricultural and Biological Sciences | 1 | 20% |