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Protein Gel Detection and Imaging

Overview of attention for book
Cover of 'Protein Gel Detection and Imaging'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Protein Stains and Applications
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    Chapter 2 The Roles of Acetic Acid and Methanol During Fixing and Staining Proteins in an SDS–Polyacrylamide Electrophoresis Gel
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    Chapter 3 Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of Protein Bands During SDS-PAGE
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    Chapter 4 Coomassie Brilliant Blue Staining of Polyacrylamide Gels
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    Chapter 5 A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Using Coomassie Blue
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    Chapter 6 Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue
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    Chapter 7 Silver Staining Techniques of Polyacrylamide Gels
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    Chapter 8 Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry
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    Chapter 9 Detection of Phosphoproteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using 8-Quinolinol Stain
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    Chapter 10 Microwave-Assisted Protein Staining, Destaining, and In-Gel/In-Solution Digestion of Proteins
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    Chapter 11 Fluorescent Staining of Gels
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    Chapter 12 A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis
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    Chapter 13 TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE
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    Chapter 14 Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain
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    Chapter 15 Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain
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    Chapter 16 Detection of Multiple Enzymes in Fermentation Broth Using Single PAGE Analysis
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    Chapter 17 Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis
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    Chapter 18 A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins
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    Chapter 19 Ten Minute Stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black
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    Chapter 20 In-Gel Protein Phosphatase Assay Using Fluorogenic Substrates
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    Chapter 21 Detection of Proteins in Polyacrylamide Gels via Prelabeling by Isatoic Anhydride
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    Chapter 22 Fluorescent Protein Visualization Immediately After Gel Electrophoresis Using an In-Gel Trichloroethanol Photoreaction with Tryptophan
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    Chapter 23 Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel
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    Chapter 24 Zymographic Determination of Intrinsic Specific Activity of Oxidases in the Presence of Interfering Proteins
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    Chapter 25 A Simple Method for Detecting Phosphorylation of Proteins by Using Zn2+-Phos-Tag SDS-PAGE at Neutral pH
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    Chapter 26 Principle and Method of Silver Staining of Proteins Separated by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis
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    Chapter 27 Heat/Pressure Treatment with Detergents Significantly Increases Curcumin Solubility and Stability: Its Use as an Environment-Friendly Protein Gel Stain
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    Chapter 28 Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels
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    Chapter 29 Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate–Polyacrylamide Protein Gels Using a Household Detergent
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    Chapter 30 Paper Adsorbents Remove Coomassie Blue from Gel Destain and Used Gel Stain in an Environment-Friendly Manner
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    Chapter 31 Gel Drying Methods
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    Chapter 32 Stained Gels Can Be Stored for Several Months in Nonsealed Polyethylene Bags
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    Chapter 33 Radiolabeling and Analysis of Labeled Proteins
Attention for Chapter 14: Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain
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Chapter title
Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain
Chapter number 14
Book title
Protein Gel Detection and Imaging
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8745-0_14
Pubmed ID
Book ISBNs
978-1-4939-8744-3, 978-1-4939-8745-0
Authors

Mehta-D'souza, Padmaja, Padmaja Mehta-D’souza, Mehta-D’souza, Padmaja

Abstract

Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic-acid Schiff's base which uses acidic fuchsin dye.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 20%
Researcher 2 13%
Other 1 7%
Student > Bachelor 1 7%
Librarian 1 7%
Other 4 27%
Unknown 3 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 33%
Agricultural and Biological Sciences 2 13%
Medicine and Dentistry 2 13%
Nursing and Health Professions 1 7%
Social Sciences 1 7%
Other 1 7%
Unknown 3 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 August 2018.
All research outputs
#15,542,971
of 23,099,576 outputs
Outputs from Methods in molecular biology
#5,412
of 13,208 outputs
Outputs of similar age
#270,171
of 442,686 outputs
Outputs of similar age from Methods in molecular biology
#596
of 1,499 outputs
Altmetric has tracked 23,099,576 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,686 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.