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Plant Chemical Genomics

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Cover of 'Plant Chemical Genomics'

Table of Contents

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    Book Overview
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    Chapter 1 Multi-Parametric Screening in Arabidopsis thaliana Seedlings
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    Chapter 2 Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress
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    Chapter 3 Method for Ultrarapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen
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    Chapter 4 A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death Using Suspension Cell Culture of Arabidopsis thaliana
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    Chapter 5 High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression
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    Chapter 6 Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana Using Chemical Genetics
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    Chapter 7 Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls Using BY-2 Cells
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    Chapter 8 Small-Molecule Screening to Increase Agrobacterium -Mediated Transformation Efficiency in Legumes
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    Chapter 9 High-Throughput In Vitro Screening for Inhibitors of Cereal α-Glucosidase
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    Chapter 10 Chemical Screening for Strigolactone Receptor Antagonists Using Arabidopsis thaliana
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    Chapter 11 Chemical Control of ABA Receptors to Enable Plant Protection Against Water Stress
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    Chapter 12 Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening
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    Chapter 13 Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside That Controls the Leaf Closure of Samanea saman
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    Chapter 14 Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule
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    Chapter 15 Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants Using PlantiSMASH
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    Chapter 16 Chemical Genomics Translatability from Unicellular to Multicellular Models
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    Chapter 17 Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii
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    Chapter 18 FnCpf1-Mediated Targeted Mutagenesis in Plants
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    Chapter 19 Erratum to: Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii
Attention for Chapter 18: FnCpf1-Mediated Targeted Mutagenesis in Plants
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Chapter title
FnCpf1-Mediated Targeted Mutagenesis in Plants
Chapter number 18
Book title
Plant Chemical Genomics
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7874-8_18
Pubmed ID
Book ISBNs
978-1-4939-7873-1, 978-1-4939-7874-8
Authors

Akira Endo, Seiichi Toki, Endo, Akira, Toki, Seiichi

Abstract

Sequence-specific nucleases (SSNs) are nowadays fundamental tools to generate mutants that impaired in genes of interest. The bioactive molecules screened in the chemical genomics studies affect specific physiological process by disrupting the function of its target protein(s). Mutation analysis of the gene(s) of target protein(s) of the screened chemical is necessary to resolve how the chemical works in plants. Clustered regularly interspersed short palindromic repeats (CRISPR) from Prevotella and Francisella 1 (Cpf1) are newly characterized RNA-directed endonuclease. Several papers have shown clearly that Cpf1 could be a versatile SSN in plant genome engineering. Cfp1 from Francisella novicida (FnCpf1) recognizes TTN as its protospacer adjacent motif (PAM). FnCpf1 utilizes a shorter PAM compared to other known Cpf1s such as AsCpf1 or LbCpf1, which use TTTN as PAM. Since PAM length can be a limiting factor in target selection, this feature of FnCpf1 is practical for targeted mutagenesis experiments. The application of FnCpf1-mediated targeted mutagenesis to the chemical genomics could accelerate to figure out the mechanism of action of screened chemicals. Here, we describe procedures for targeted mutagenesis in rice and tobacco using FnCpf1.

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Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 63%
Unspecified 1 13%
Researcher 1 13%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 50%
Agricultural and Biological Sciences 2 25%
Unspecified 1 13%
Engineering 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2018.
All research outputs
#20,516,195
of 23,083,773 outputs
Outputs from Methods in molecular biology
#9,974
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Outputs of similar age
#378,449
of 442,614 outputs
Outputs of similar age from Methods in molecular biology
#1,194
of 1,499 outputs
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