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Plant Transcription Factors

Overview of attention for book
Cover of 'Plant Transcription Factors'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
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    Chapter 2 Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
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    Chapter 3 The Long-Term “In Natura” Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
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    Chapter 4 Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
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    Chapter 5 A Specific Knockdown of Transcription Factor Activities in Arabidopsis
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    Chapter 6 Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
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    Chapter 7 CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
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    Chapter 8 Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
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    Chapter 9 Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
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    Chapter 10 Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
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    Chapter 11 High Impact Gene Discovery: Simple Strand-Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
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    Chapter 12 Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
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    Chapter 13 NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
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    Chapter 14 Genome-Wide TSS Identification in Maize
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    Chapter 15 Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
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    Chapter 16 Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci
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    Chapter 17 Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines
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    Chapter 18 Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA-Binding Interactions and Identify cis-Elements
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    Chapter 19 In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins
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    Chapter 20 Cell-Free Protein Synthesis of Plant Transcription Factors
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    Chapter 21 Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants
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    Chapter 22 Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
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    Chapter 23 Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants
Attention for Chapter 6: Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
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Chapter title
Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
Chapter number 6
Book title
Plant Transcription Factors
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8657-6_6
Pubmed ID
Book ISBNs
978-1-4939-8656-9, 978-1-4939-8657-6
Authors

Ze Hong Lee, Nobutoshi Yamaguchi, Toshiro Ito, Lee, Ze Hong, Yamaguchi, Nobutoshi, Ito, Toshiro

Abstract

Gene editing enables the modification of genetic information in a targeted manner. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a gene editing approach that has been widely used in both the animal and plant studies. Plant transcription factors play critical roles in plant development. Recent studies have used the CRISPER/Cas9 system to disrupt transcription factors and cis-elements in plants. In this chapter, we describe a protocol of using the CRISPR/Cas9 system to generate transcription factor mutants in the model plant species Arabidopsis thaliana. Our protocol contains five different steps: (1) adaptor primers design, (2) cloning of sgRNA expression cassettes, (3) Golden Gate cloning, (4) E. coli and Agrobacterium transformation, and (5) Arabidopsis transformation.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 49 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 11 22%
Student > Ph. D. Student 7 14%
Student > Master 4 8%
Student > Doctoral Student 2 4%
Researcher 2 4%
Other 1 2%
Unknown 22 45%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 21 43%
Agricultural and Biological Sciences 3 6%
Environmental Science 1 2%
Unspecified 1 2%
Neuroscience 1 2%
Other 1 2%
Unknown 21 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 July 2018.
All research outputs
#15,290,235
of 24,712,008 outputs
Outputs from Methods in molecular biology
#4,301
of 13,879 outputs
Outputs of similar age
#244,267
of 453,055 outputs
Outputs of similar age from Methods in molecular biology
#409
of 1,486 outputs
Altmetric has tracked 24,712,008 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,879 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 453,055 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,486 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.