↓ Skip to main content

Plant Transcription Factors

Overview of attention for book
Cover of 'Plant Transcription Factors'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
  3. Altmetric Badge
    Chapter 2 Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
  4. Altmetric Badge
    Chapter 3 The Long-Term “In Natura” Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
  5. Altmetric Badge
    Chapter 4 Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
  6. Altmetric Badge
    Chapter 5 A Specific Knockdown of Transcription Factor Activities in Arabidopsis
  7. Altmetric Badge
    Chapter 6 Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
  8. Altmetric Badge
    Chapter 7 CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
  9. Altmetric Badge
    Chapter 8 Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
  10. Altmetric Badge
    Chapter 9 Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
  11. Altmetric Badge
    Chapter 10 Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
  12. Altmetric Badge
    Chapter 11 High Impact Gene Discovery: Simple Strand-Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
  13. Altmetric Badge
    Chapter 12 Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
  14. Altmetric Badge
    Chapter 13 NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
  15. Altmetric Badge
    Chapter 14 Genome-Wide TSS Identification in Maize
  16. Altmetric Badge
    Chapter 15 Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
  17. Altmetric Badge
    Chapter 16 Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci
  18. Altmetric Badge
    Chapter 17 Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines
  19. Altmetric Badge
    Chapter 18 Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA-Binding Interactions and Identify cis-Elements
  20. Altmetric Badge
    Chapter 19 In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins
  21. Altmetric Badge
    Chapter 20 Cell-Free Protein Synthesis of Plant Transcription Factors
  22. Altmetric Badge
    Chapter 21 Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants
  23. Altmetric Badge
    Chapter 22 Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
  24. Altmetric Badge
    Chapter 23 Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants
Attention for Chapter 4: Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
Altmetric Badge

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
10 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
Chapter number 4
Book title
Plant Transcription Factors
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8657-6_4
Pubmed ID
Book ISBNs
978-1-4939-8656-9, 978-1-4939-8657-6
Authors

Pooja Aggarwal, Krishna Reddy Challa, Monalisha Rath, Preethi Sunkara, Utpal Nath, Aggarwal, Pooja, Challa, Krishna Reddy, Rath, Monalisha, Sunkara, Preethi, Nath, Utpal

Abstract

Transcription factors play key regulatory roles in all the life processes across kingdoms. In plants, the genome of a typical model species such as Arabidopsis thaliana encodes over 1500 transcription factors that regulate the expression dynamics of all the genes in time and space. Therefore, studying their function by analyzing the loss and gain-of-function lines is of prime importance in basic plant biology and its agricultural application. However, the current approach of knocking out genes often causes embryonic lethal phenotype, while inactivating one or two members of a redundant gene family yields little phenotypic changes, thereby making the functional analysis a technically challenging task. In such cases, inducible knock-down or overexpression of transcription factors appears to be a more effective approach. Restricting the transcription factors in the cytoplasm by fusing them with animal glucocorticoid/estrogen receptors (GR/ER) and then re-localizing them to the nucleus by external application of animal hormone analogues has been a useful method of gene function analysis in the model plants. In this chapter, we describe the recent advancements in the GR and ER expression systems and their use in analyzing the function of transcription factors in Arabidopsis.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 40%
Researcher 2 20%
Student > Bachelor 2 20%
Unspecified 1 10%
Unknown 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 40%
Biochemistry, Genetics and Molecular Biology 3 30%
Unspecified 1 10%
Immunology and Microbiology 1 10%
Unknown 1 10%