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Plant Transcription Factors

Overview of attention for book
Cover of 'Plant Transcription Factors'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
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    Chapter 2 Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
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    Chapter 3 The Long-Term “In Natura” Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
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    Chapter 4 Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
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    Chapter 5 A Specific Knockdown of Transcription Factor Activities in Arabidopsis
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    Chapter 6 Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
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    Chapter 7 CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
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    Chapter 8 Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
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    Chapter 9 Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
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    Chapter 10 Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
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    Chapter 11 High Impact Gene Discovery: Simple Strand-Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
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    Chapter 12 Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
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    Chapter 13 NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
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    Chapter 14 Genome-Wide TSS Identification in Maize
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    Chapter 15 Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
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    Chapter 16 Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci
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    Chapter 17 Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines
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    Chapter 18 Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA-Binding Interactions and Identify cis-Elements
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    Chapter 19 In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins
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    Chapter 20 Cell-Free Protein Synthesis of Plant Transcription Factors
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    Chapter 21 Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants
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    Chapter 22 Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
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    Chapter 23 Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants
Attention for Chapter 7: CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
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  • High Attention Score compared to outputs of the same age and source (91st percentile)

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Chapter title
CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
Chapter number 7
Book title
Plant Transcription Factors
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8657-6_7
Pubmed ID
Book ISBNs
978-1-4939-8656-9, 978-1-4939-8657-6
Authors

Shigeo S. Sugano, Ryuichi Nishihama, Sugano, Shigeo S., Nishihama, Ryuichi

Abstract

Plant transcription factors (TFs) belong to a wide variety of gene families. The systematic and rapid establishment of knockout lines of TF genes is critical for functional genetics. Genome engineering techniques for dissecting out the molecular function of TFs have been dramatically improved by CRISPR/Cas9-based genome editing technology. In the CRISPR/Cas9 system, Cas9 functions as a Cas9-gRNA ribonucleoprotein complex and uses its DNA endonuclease activity to induce the cleavage of the genome, which is targeted by gRNA. Double-strand breaks sometimes induce insertions and deletions at the target site, leading to frameshift mutations of TF genes. In this chapter, we describe a detailed protocol for the targeted mutagenesis of TFs using the CRISPR/Cas9 system, specifically in the case of Marchantia polymorpha, an emerging model plant for functional genomics. The CRISPR/Cas9 system is highly versatile for targeting genomic sequences because only an alteration of the gRNA sequence is needed to change target sequences. The labor and cost required to establish genome-edited lines are low enough that multiple mutants of TF genes can be generated in one laboratory. The CRISPR/Cas9-based genome editing technique consists of four steps: (1) gRNA design; (2) vector construction; (3) transformation; and (4) isolation of genome-edited lines. This manuscript focuses mainly on the strategy of gRNA design, the workflow for off-target searches, and the selection and identification of genome-edited lines by genotyping. Although we describe a protocol for M. polymorpha, the basic strategy of generating genome-edited lines of TF genes should be applicable widely to other plants.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 45 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 20%
Student > Master 6 13%
Student > Bachelor 6 13%
Researcher 5 11%
Student > Doctoral Student 1 2%
Other 3 7%
Unknown 15 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 16 36%
Agricultural and Biological Sciences 10 22%
Unspecified 1 2%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Neuroscience 1 2%
Other 2 4%
Unknown 14 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2018.
All research outputs
#4,776,781
of 23,096,849 outputs
Outputs from Methods in molecular biology
#1,392
of 13,208 outputs
Outputs of similar age
#107,045
of 442,670 outputs
Outputs of similar age from Methods in molecular biology
#127
of 1,499 outputs
Altmetric has tracked 23,096,849 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,670 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.