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Plant Transcription Factors

Overview of attention for book
Cover of 'Plant Transcription Factors'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
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    Chapter 2 Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
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    Chapter 3 The Long-Term “In Natura” Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
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    Chapter 4 Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
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    Chapter 5 A Specific Knockdown of Transcription Factor Activities in Arabidopsis
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    Chapter 6 Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
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    Chapter 7 CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
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    Chapter 8 Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
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    Chapter 9 Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
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    Chapter 10 Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
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    Chapter 11 High Impact Gene Discovery: Simple Strand-Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
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    Chapter 12 Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
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    Chapter 13 NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
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    Chapter 14 Genome-Wide TSS Identification in Maize
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    Chapter 15 Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
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    Chapter 16 Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci
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    Chapter 17 Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines
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    Chapter 18 Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA-Binding Interactions and Identify cis-Elements
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    Chapter 19 In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins
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    Chapter 20 Cell-Free Protein Synthesis of Plant Transcription Factors
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    Chapter 21 Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants
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    Chapter 22 Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
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    Chapter 23 Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants
Attention for Chapter 13: NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
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Chapter title
NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
Chapter number 13
Book title
Plant Transcription Factors
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8657-6_13
Pubmed ID
Book ISBNs
978-1-4939-8656-9, 978-1-4939-8657-6
Authors

Maria G. Ivanchenko, Molly Megraw, Ivanchenko, Maria G., Megraw, Molly

Abstract

Identifying the transcription start sites (TSS) of genes is essential for characterizing promoter regions. Several protocols have been developed to capture the 5' end of transcripts via Cap-Analysis of Gene Expression (CAGE) or linker-ligation strategies such as Paired-End Analysis of Transcription Start Sites (PEAT), but often require large amounts of tissue. More recently, nanoCAGE was developed for sequencing on the Illumina GAIIx to overcome this limitation. In this chapter, we present the nanoCAGE-XL protocol, the first publicly available adaptation of nanoCAGE for sequencing on recent ultra-high-throughput platforms such as Illumina HiSeq-2000. NanoCAGE-XL provides a method for precise transcription start site identification in large eukaryotic genomes, even in cases where input total RNA quantity is very limited.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 40%
Researcher 4 40%
Other 1 10%
Student > Master 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 50%
Biochemistry, Genetics and Molecular Biology 3 30%
Arts and Humanities 1 10%
Medicine and Dentistry 1 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 July 2018.
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#18,643,992
of 23,096,849 outputs
Outputs from Methods in molecular biology
#7,989
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#330,888
of 442,670 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,499 outputs
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