Chapter title |
The Impact of Lipophilicity in Drug Discovery: Rapid Measurements by Means of Reversed-Phase HPLC
|
---|---|
Chapter number | 12 |
Book title |
Rational Drug Design
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8630-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8629-3, 978-1-4939-8630-9
|
Authors |
Constantinos Giaginis, Fotios Tsopelas, Anna Tsantili-Kakoulidou, Giaginis, Constantinos, Tsopelas, Fotios, Tsantili-Kakoulidou, Anna |
Abstract |
Lipophilicity constitutes a vital physicochemical property in drug design as it is connected with pharmacodynamic and pharmacokinetic properties as well as toxicological aspects of candidate drugs. Traditional partitioning experiments to determine n-octanol-water coefficients are laborious and time-consuming, while they cannot be reliably performed for highly lipophilic or compounds undergoing degradation. Alternatively, lipophilicity of candidate drugs can be accurately and reproducibly determined using reversed-phase liquid chromatography. In this chapter, the details of protocols for lipophilicity assessment using reversed-phase HPLC, under conditions which provide the best simulation of n-octanol-water partition coefficients, are described. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 18% |
Researcher | 2 | 18% |
Student > Master | 2 | 18% |
Student > Bachelor | 1 | 9% |
Other | 1 | 9% |
Other | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 2 | 18% |
Nursing and Health Professions | 1 | 9% |
Unspecified | 1 | 9% |
Immunology and Microbiology | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Other | 0 | 0% |
Unknown | 5 | 45% |