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Nucleic Acid Crystallography

Overview of attention for book
Cover of 'Nucleic Acid Crystallography'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Perspectives and Pitfalls in Nucleic Acids Crystallography
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    Chapter 2 Nucleic Acid Crystallography
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    Chapter 3 Preparation and Crystallization of Riboswitches
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    Chapter 4 In Vitro/In Vivo Production of tRNA for X-Ray Studies
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    Chapter 5 Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA
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    Chapter 6 Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs
  8. Altmetric Badge
    Chapter 7 Nucleic Acid Crystallography
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    Chapter 8 Nucleic Acid Crystallography
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    Chapter 9 Isothermal Titration Calorimetry: Assisted Crystallization of RNA–Ligand Complexes
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    Chapter 10 Crystallographic Data and Model Quality
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    Chapter 11 Advanced Crystallographic Data Collection Protocols for Experimental Phasing
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    Chapter 12 Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases
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    Chapter 13 Practical Radiation Damage-Induced Phasing
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    Chapter 14 Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data
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    Chapter 15 Using Molecular Replacement Phasing to Study the Structure and Function of RNA
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    Chapter 16 Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?
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    Chapter 17 RNA Structure Refinement Using the ERRASER-Phenix Pipeline
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    Chapter 18 Neutron Nucleic Acid Crystallography
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    Chapter 19 Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.
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    Chapter 20 Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers.
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    Chapter 21 Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor.
  23. Altmetric Badge
    Chapter 22 Nucleic Acid Crystallography
Attention for Chapter 19: Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.
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Chapter title
Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.
Chapter number 19
Book title
Nucleic Acid Crystallography
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-2763-0_19
Pubmed ID
Book ISBNs
978-1-4939-2762-3, 978-1-4939-2763-0
Authors

Agalarov, Sultan, Yusupov, Marat, Yusupova, Gulnara, Sultan Agalarov, Marat Yusupov, Gulnara Yusupova

Abstract

In vitro reconstitution systems of ribosomal subunits from free ribosomal RNA and ribosomal proteins are helpful tool for studies on the structure, function and assembly of ribosome. Using this system mutant or modified ribosomal proteins or ribosomal RNA can be incorporated into ribosomal subunits for studying ribosome structure and function. Developing the protocol for reconstitution of 30S subunits from an extreme thermophilic bacterium Thermus thermophilus can be beneficial especially for structural studies, as proteins and nucleic acids from this organism are very stable and crystallize easier than those from mesophilic organisms.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 17%
Unknown 5 83%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 33%
Student > Ph. D. Student 1 17%
Professor 1 17%
Unknown 2 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 33%
Biochemistry, Genetics and Molecular Biology 1 17%
Engineering 1 17%
Unknown 2 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 August 2015.
All research outputs
#15,340,815
of 22,818,766 outputs
Outputs from Methods in molecular biology
#5,343
of 13,121 outputs
Outputs of similar age
#230,835
of 393,497 outputs
Outputs of similar age from Methods in molecular biology
#545
of 1,470 outputs
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So far Altmetric has tracked 13,121 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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