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Two-Hybrid Systems

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Cover of 'Two-Hybrid Systems'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Protein Interactomics by Two-Hybrid Methods
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    Chapter 2 Making the Right Choice: Critical Parameters of the Y2H Systems
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    Chapter 3 Identification of Protein–Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening
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    Chapter 4 Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System
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    Chapter 5 The Ras Recruitment System (RRS) for the Identification and Characterization of Protein–Protein Interactions
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    Chapter 6 A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway ® Cloning
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    Chapter 7 Analyses of Protein Interaction Networks Using Computational Tools
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    Chapter 8 Options and Considerations When Using a Yeast One-Hybrid System
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    Chapter 9 Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins
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    Chapter 10 Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein–DNA Interactions by High-Throughput Yeast One-Hybrid Screens
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    Chapter 11 Transcription Factor-Centered Yeast One-Hybrid Assay
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    Chapter 12 The Yeast Three-Hybrid System for Protein Interactions
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    Chapter 13 The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants
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    Chapter 14 Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules
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    Chapter 15 Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System
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    Chapter 16 Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein–Protein Interactions in Plant Cells
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    Chapter 17 Bimolecular Fluorescence Complementation to Visualize Protein–Protein Interactions in Human Cells Based on Gateway Cloning Technology
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    Chapter 18 KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein–Protein Interactions
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    Chapter 19 Coimmunoprecipitation of Interacting Proteins in Plants
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    Chapter 20 Analysis of Protein–Protein Interaction by Co-IP in Human Cells
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    Chapter 21 Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures
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    Chapter 22 Transient Transactivation Studies in Nicotiana benthamiana Leaves
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    Chapter 23 The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein–DNA Interactions
  25. Altmetric Badge
    Chapter 24 Chromatin Immunoprecipitation for Identification of Protein–DNA Interactions in Human Cells
Attention for Chapter 9: Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins
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Chapter title
Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins
Chapter number 9
Book title
Two-Hybrid Systems
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7871-7_9
Pubmed ID
Book ISBNs
978-1-4939-7870-0, 978-1-4939-7871-7
Authors

Rocío Sánchez-Montesino, Luis Oñate-Sánchez, Sánchez-Montesino, Rocío, Oñate-Sánchez, Luis

Abstract

Molecular interactions are an integral part of the regulatory mechanisms controlling gene expression. The yeast one- and two-hybrid systems (Y1H/Y2H) have been widely used by many laboratories to detect DNA-protein (Y1H) and protein-protein interactions (Y2H). The development of efficient cloning systems have promoted the generation of large open reading frame (ORF) clone collections (libraries) for several organisms. Functional analyses of such large collections require the establishment of adequate protocols. Here, we describe a simple straightforward procedure for high-throughput screenings of arrayed libraries with DNA or protein baits that can be carried out by one person with minimal labor and not requiring robotics. The protocol can also be scaled up or down and is compatible with several library formats. Procedures to make yeast stocks for long-term storage (tube and microplate formats) are also provided.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 22%
Researcher 1 11%
Student > Master 1 11%
Unknown 5 56%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 11%
Agricultural and Biological Sciences 1 11%
Immunology and Microbiology 1 11%
Unknown 6 67%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2018.
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#20,518,141
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Outputs from Methods in molecular biology
#9,974
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#378,445
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Outputs of similar age from Methods in molecular biology
#1,194
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