| Chapter title |
Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells
|
|---|---|
| Chapter number | 18 |
| Book title |
Mitochondrial Bioenergetics
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7831-1_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7830-4, 978-1-4939-7831-1
|
| Authors |
Shun-ichi Yamashita, Tomotake Kanki, Yamashita, Shun-ichi, Kanki, Tomotake |
| Abstract |
Mitochondrial autophagy or mitophagy is a process that selectively degrades mitochondria via autophagy. It is believed that mitophagy degrades damaged or unnecessary mitochondria and is important for maintaining mitochondrial homeostasis. To date, it is known that several stimuli can induce mitophagy. However, some of these stimuli (including iron depletion, hypoxia, and nitrogen starvation) induce mild mitophagy, which is difficult to detect by measuring the decrease in mitochondrial mass. Recently, we have successfully detected mitophagy induced under these conditions using mito-Keima as a reporter. In this chapter, we describe the protocols for induction and detection of iron depletion- and hypoxia-induced mitophagy using the mito-Keima-expressing cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 45 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 24% |
| Researcher | 9 | 20% |
| Student > Bachelor | 4 | 9% |
| Other | 2 | 4% |
| Student > Postgraduate | 2 | 4% |
| Other | 4 | 9% |
| Unknown | 13 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 24% |
| Agricultural and Biological Sciences | 5 | 11% |
| Arts and Humanities | 3 | 7% |
| Neuroscience | 3 | 7% |
| Medicine and Dentistry | 3 | 7% |
| Other | 5 | 11% |
| Unknown | 15 | 33% |