Chapter title |
A Method for Prostate and Breast Cancer Cell Spheroid Cultures Using Gelatin Methacryloyl-Based Hydrogels
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Chapter number | 10 |
Book title |
Prostate Cancer
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7845-8_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7843-4, 978-1-4939-7845-8
|
Authors |
Christoph Meinert, Christina Theodoropoulos, Travis J. Klein, Dietmar W. Hutmacher, Daniela Loessner, Meinert, Christoph, Theodoropoulos, Christina, Klein, Travis J., Hutmacher, Dietmar W., Loessner, Daniela |
Abstract |
Modern tissue engineering technologies have delivered tools to recreate a cell's naturally occurring niche in vitro and to investigate normal and pathological cell-cell and cell-niche interactions. Hydrogel biomaterials mimic crucial properties of native extracellular matrices, including mechanical support, cell adhesion sites and proteolytic degradability. As such, they are applied as 3D cell culture platforms to replicate tissue-like architectures observed in vivo, allowing physiologically relevant cell behaviors. Here we review bioengineered 3D approaches used for prostate and breast cancer. Furthermore, we describe the synthesis and use of gelatin methacryloyl-based hydrogels as in vitro 3D cancer model. This platform is used to engineer the microenvironments for prostate and breast cancer cells to study processes regulating spheroid formation, cell functions and responses to therapeutic compounds. Collectively, these bioengineered 3D approaches provide cell biologists with innovative pre-clinical tools that integrate the complexity of the disease seen in patients to advance our knowledge of cancer cell physiology and the contribution of a tumor's surrounding milieu. |
X Demographics
Geographical breakdown
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Italy | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 53 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 17% |
Researcher | 9 | 17% |
Student > Master | 8 | 15% |
Student > Doctoral Student | 3 | 6% |
Student > Bachelor | 3 | 6% |
Other | 6 | 11% |
Unknown | 15 | 28% |
Readers by discipline | Count | As % |
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Engineering | 11 | 21% |
Medicine and Dentistry | 5 | 9% |
Biochemistry, Genetics and Molecular Biology | 5 | 9% |
Materials Science | 4 | 8% |
Agricultural and Biological Sciences | 2 | 4% |
Other | 6 | 11% |
Unknown | 20 | 38% |