Article
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Attention Score in Context
| Chapter title |
Isolation and Phenotyping of Adult Mouse Microglial Cells
|
|---|---|
| Chapter number | 7 |
| Book title |
Macrophages
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7837-3_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7836-6, 978-1-4939-7837-3
|
| Authors |
Kathleen Grabert, Barry W. McColl, Grabert, Kathleen, McColl, Barry W. |
| Abstract |
Microglia are the resident macrophages of the central nervous system parenchyma and fulfill crucial roles in brain development, homeostasis, and inflammation. The isolation of a pure microglia population from brain tissue enables the examination of microglial phenotypes without the interference of other cell populations. Microglial extractions from the neonatal brain have been described in various protocols, yet the more established and complex adult mouse brain poses a greater challenge. Here we describe a refined protocol including enzymatic and mechanical dissociation of adult mouse brain tissue and removal of myelin by Percoll density gradient. Microglial cells were subsequently extracted by an immunomagnetic approach. This isolation procedure enables the use of functionally viable cells for various applications such as cell culture, flow cytometry, functional assays including bacteria- or bead-based phagocytosis, stimulation assays, and transcriptome profiling techniques such as qRT-PCR and microarray/RNA sequencing. |
X Demographics
The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 11 | 50% |
| United States | 3 | 14% |
| Canada | 1 | 5% |
| Brazil | 1 | 5% |
| Unknown | 6 | 27% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 11 | 50% |
| Scientists | 9 | 41% |
| Practitioners (doctors, other healthcare professionals) | 2 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 90 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 23% |
| Researcher | 14 | 16% |
| Student > Master | 10 | 11% |
| Student > Bachelor | 7 | 8% |
| Student > Doctoral Student | 5 | 6% |
| Other | 9 | 10% |
| Unknown | 24 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 19 | 21% |
| Biochemistry, Genetics and Molecular Biology | 13 | 14% |
| Medicine and Dentistry | 9 | 10% |
| Agricultural and Biological Sciences | 7 | 8% |
| Immunology and Microbiology | 6 | 7% |
| Other | 11 | 12% |
| Unknown | 25 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2019.
All research outputs
#2,381,433
of 23,055,429 outputs
Outputs from Methods in molecular biology
#426
of 13,196 outputs
Outputs of similar age
#56,855
of 442,477 outputs
Outputs of similar age from Methods in molecular biology
#23
of 1,499 outputs
Altmetric has tracked 23,055,429 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,196 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,477 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.