Chapter title |
Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells
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Chapter number | 10 |
Book title |
Circulating Tumor Cells
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7144-2_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7143-5, 978-1-4939-7144-2
|
Authors |
Marianne Oulhen, Emma Pailler, Vincent Faugeroux, Françoise Farace, Oulhen, Marianne, Pailler, Emma, Faugeroux, Vincent, Farace, Françoise |
Abstract |
Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FISH protocol, the combination of immunofluorescent staining (DAPI/CD45) and FA-FISH techniques, as well as the semi-automated microscopy method that we developed to improve the feasibility and reliability of FISH analyses in filtration-enriched CTC. |
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