Rab GTPases
Springer New York
Chapter title |
Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells with or without NGF.
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Chapter number | 25 |
Book title |
Rab GTPases
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2569-8_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2568-1, 978-1-4939-2569-8
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Authors |
M Caleb Marlin, Guangpu Li, M. Caleb Marlin, Marlin, M. Caleb, Li, Guangpu |
Abstract |
Macroautophagy selectively recycles damaged or unneeded proteins and organelles by degradation via targeting to the autophagosome. The following method seeks to identify candidate Rab GTPases that likely modulate autophagy in PC12 cells during nerve growth factor (NGF) starvation. This microscopy-based assay is a single cell-based quantification of the presence of autophagosomes by fluorescently labeled markers in response to the overexpression of Rabs and mutants in the presence or absence of NGF. |
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Student > Bachelor | 2 | 67% |
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Biochemistry, Genetics and Molecular Biology | 2 | 67% |
Unknown | 1 | 33% |