Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants…
Springer New York
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Timeline
Mendeley readers
| Chapter title |
In Vitro Shoot Cultures and Analysis of Steroidal Lactones in Withania coagulans (Stocks) Dunal
|
|---|---|
| Chapter number | 18 |
| Book title |
Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3332-7_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3330-3, 978-1-4939-3332-7
|
| Authors |
Rohit Jain, Sumita Kachhwaha, S. L. Kothari, Jain, Rohit, Kachhwaha, Sumita, Kothari, S. L. |
| Abstract |
Withania coagulans (Stocks) Dunal (Solanaceae), also known as 'Panir Bandh' is an important medicinal plant that is extensively used as a home remedy for several diseases in the Indian subcontinent. The plant possesses specific steroidal lactones known as withanolides which show high level of pharmaceutical activity against a broad spectrum of microorganisms. Natural propagation of the plant occurs through Seed but due to unisexual nature of the flowers; chances of Seed setting are very limited and the plant is on the verge of extinction because of overexploitation and reproductive failure. Plant tissue culture techniques offer opportunities for ex situ conservation and mass multiplication of endangered plant species through micropropagation and also enhancement of in vitro biosynthesis of bioactive compounds. In this chapter we present protocols for the mass multiplication of W. coagulans, assessment of clonal fidelity by RAPD, and estimation of bioactive compounds (withanolides) by thin layer chromatography (TLC) and reverse phase HPLC developed in our laboratory. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 25% |
| Student > Postgraduate | 2 | 13% |
| Researcher | 2 | 13% |
| Student > Ph. D. Student | 1 | 6% |
| Other | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 19% |
| Environmental Science | 2 | 13% |
| Chemistry | 2 | 13% |
| Medicine and Dentistry | 2 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 31% |