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Mendeley readers
Chapter title |
Supported Molecular Matrix Electrophoresis
|
---|---|
Chapter number | 31 |
Book title |
Detection of Blotted Proteins
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2718-0_31 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2717-3, 978-1-4939-2718-0
|
Authors |
Yu-ki Matsuno, Akihiko Kameyama, Matsuno, Yu-ki, Kameyama, Akihiko |
Abstract |
Mucins are difficult to separate using conventional gel electrophoresis methods such as SDS-PAGE and agarose gel electrophoresis, owing to their large size and heterogeneity. On the other hand, cellulose acetate membrane electrophoresis can separate these molecules, but is not compatible with glycan analysis. Here, we describe a novel membrane electrophoresis technique, termed "supported molecular matrix electrophoresis" (SMME), in which a porous polyvinylidene difluoride (PVDF) membrane filter is used to achieve separation. This description includes the separation, visualization, and glycan analysis of mucins with the SMME technique. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 25% |
Unknown | 3 | 75% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 1 | 25% |
Unknown | 3 | 75% |