Schizosaccharomyces pombe
Springer New York
Chapter title |
The No-Nonsens SDS-PAGE
|
---|---|
Chapter number | 8 |
Book title |
Schizosaccharomyces pombe
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7546-4_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7545-7, 978-1-4939-7546-4
|
Authors |
Lars Backman, Karina Persson, Backman, Lars, Persson, Karina |
Abstract |
The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680-685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin. |
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