Chapter title |
A Brief Guide to the High-Throughput Expression of Directed Evolution Libraries
|
---|---|
Chapter number | 7 |
Book title |
Protein Engineering
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7366-8_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7364-4, 978-1-4939-7366-8
|
Authors |
Ana Luísa Ribeiro, Mario Mencía, Aurelio Hidalgo, Ribeiro, Ana Luísa, Mencía, Mario, Hidalgo, Aurelio |
Abstract |
The process of protein production optimization requires time and labor, constituting one of the main bottlenecks for the downstream utilization of the proteins. However, once through this bottleneck, the protein production process can be easily standardized and multiplexed to find the fittest variants in large libraries created by random mutagenesis. In this chapter, we present an overview of the most important choices to achieve homogeneous and functional expression of directed evolution libraries in microplate format: (1) choice of induction system and host strain, (2) choice of media and growth conditions, and (3) modifications to the genetic sequence. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 46% |
Student > Ph. D. Student | 1 | 8% |
Unspecified | 1 | 8% |
Professor > Associate Professor | 1 | 8% |
Student > Master | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 31% |
Agricultural and Biological Sciences | 3 | 23% |
Unspecified | 1 | 8% |
Engineering | 1 | 8% |
Unknown | 4 | 31% |