Chapter title |
Preparation and Physical Characterization of DNA-Binding Cationic Liposomes
|
---|---|
Chapter number | 19 |
Book title |
Liposomes
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6591-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6589-2, 978-1-4939-6591-5
|
Authors |
Vaibhav Saxena, Saxena, Vaibhav |
Abstract |
DNA-binding cationic liposomes are routinely employed as one of the major non-viral transfecting agents delivering DNA and other genes inside the cells. Cationic liposomes when complexed with DNA form a strong positively charged liposome-DNA complex or lipoplex. The chapter discusses primarily the major preparation technique for cationic liposomes and its physical characterization, with a focus on SYBR Green-I dye exclusion assay and DNA encapsulation enhancement by freeze-thaw technique. SYBR Green-I dye exclusion assay is a technique to determine the total amount of liposomal lipids required to bind a unit weight of DNA, which is critical for transfection experiments. Freeze-thaw technique on the other hand is one of the major techniques to improve DNA encapsulation in cationic liposomes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 22% |
Other | 1 | 11% |
Professor | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Student > Master | 1 | 11% |
Other | 1 | 11% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 67% |
Agricultural and Biological Sciences | 1 | 11% |
Materials Science | 1 | 11% |
Unknown | 1 | 11% |