Chapter title |
Anionic pH-Sensitive Lipoplexes
|
---|---|
Chapter number | 17 |
Book title |
Liposomes
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6591-5_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6589-2, 978-1-4939-6591-5
|
Authors |
Nathalie Mignet, Daniel Scherman, Mignet, Nathalie, Scherman, Daniel |
Abstract |
To provide long circulating nanoparticles able to carry a gene to tumors, we have designed anionic pegylated lipoplexes which are pH sensitive. Anionic pegylated lipoplexes have been prepared from the combined formulation of cationic lipoplexes and pegylated anionic liposomes. The neutralization of the particle surface charge as a function of the pH was monitored by light scattering in order to determine the ratio between anionic and cationic lipids that would give pH sensitive complexes. This ratio has been optimized to form particles sensitive to pH change in the range 5.5-6.5. Compaction of DNA into these newly formed anionic complexes is checked by DNA accessibility to picogreen. The transfection efficiency and pH sensitive property of these formulations has been shown in vitro using bafilomycin, a vacuolar H(+)-ATPase inhibitor. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 14% |
Student > Bachelor | 1 | 14% |
Researcher | 1 | 14% |
Lecturer | 1 | 14% |
Unknown | 3 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Chemistry | 1 | 14% |
Materials Science | 1 | 14% |
Engineering | 1 | 14% |
Other | 0 | 0% |
Unknown | 2 | 29% |