| Chapter title |
In-line probing analysis of riboswitches.
|
|---|---|
| Chapter number | 4 |
| Book title |
Post-Transcriptional Gene Regulation
|
| Published in |
Methods in molecular biology, March 2008
|
| DOI | 10.1007/978-1-59745-033-1_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-783-9, 978-1-59745-033-1
|
| Authors |
Regulski EE, Breaker RR, Elizabeth E. Regulski, Ronald R. Breaker, Regulski, Elizabeth E., Breaker, Ronald R. |
| Abstract |
Riboswitches are intricate, metabolite-binding RNA structures found in the non-coding regions of mRNA. Allosteric changes in the riboswitch that are induced by metabolite binding are harnessed to control the genes of a variety of essential metabolic pathways in eubacteria and in some eukaryotes. In this chapter, we describe an RNA structure analysis technique called "in-line probing." This assay makes use of the natural instability of RNA to elucidate secondary structure characteristics and ligand-binding capabilities of riboswitches in an entirely protein-free manner. Although this method has most frequently been used to examine riboswitches, the structures of any RNAs can be examined using this technique. |
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| Unknown | 274 | 94% |
Demographic breakdown
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|---|---|---|
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| Researcher | 49 | 17% |
| Student > Master | 39 | 13% |
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| Student > Doctoral Student | 17 | 6% |
| Other | 36 | 12% |
| Unknown | 30 | 10% |
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| Other | 17 | 6% |
| Unknown | 33 | 11% |