| Chapter title |
Monobodies: antibody mimics based on the scaffold of the fibronectin type III domain.
|
|---|---|
| Book title |
Protein Engineering Protocols
|
| Published in |
Methods in molecular biology, October 2006
|
| DOI | 10.1385/1-59745-187-8:95 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-072-4, 978-1-59745-187-1
|
| Authors |
Koide A, Koide S, Koide, Akiko, Koide, Shohei, Akiko Koide, Shohei Koide |
| Abstract |
We developed the use of the 10th fibronectin type III domain of human fibronectin (FNfn10) as a scaffold to display multiple surface loops for target binding. We termed FNfn10 variants with novel binding function "monobodies." FNfn10 is a small (94 residues) protein with a beta-sandwich structure similar to the immunoglobulin fold. It is highly stable without disulfide bonds or metal ions, and it can be expressed in the correctly folded form at a high level in bacteria. These desirable physical properties render the FNfn10 scaffold compatible with virtually any display technologies. This chapter describes methods for library construction and screening and for the production of monobodies. |
Mendeley readers
Geographical breakdown
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|---|---|---|
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| Unknown | 65 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Student > Master | 11 | 16% |
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|---|---|---|
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