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Peptide Libraries

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Cover of 'Peptide Libraries'

Table of Contents

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    Book Overview
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    Chapter 1 Design, Synthesis, and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries.
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    Chapter 2 Synthesis of Macrocyclic Organo-peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization.
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    Chapter 3 Synthesis and screening of one-bead-one-compound cyclic Peptide libraries.
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    Chapter 4 Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation
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    Chapter 5 Macrocyclic Templates for Library Synthesis of Peptido-Conjugates
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    Chapter 6 Synthesis and alkylation of aza-gly-pro building blocks of peptidomimetic libraries for developing prostaglandin f2α receptor modulators as therapeutics to inhibit preterm labor.
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    Chapter 7 A Fragment-Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases
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    Chapter 8 Peptide and Peptide Library Cyclization via Bromomethylbenzene Derivatives.
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    Chapter 9 High order unconditionally stable difference schemes for the Riesz space-fractional telegraph equation
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    Chapter 10 De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry
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    Chapter 11 Chemical Posttranslational Modification of Phage-Displayed Peptides
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    Chapter 12 Mapping protein-protein interactions with phage-displayed combinatorial Peptide libraries and alanine scanning.
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    Chapter 13 Identifying Reactive Peptides from Phage-Displayed Libraries
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    Chapter 14 The best and the brightest: exploiting tryptophan-sensitized tb(3+) luminescence to engineer lanthanide-binding tags.
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    Chapter 15 Synthesis and Cell-Based Screening of One-Bead-One-Compound Peptide Libraries
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    Chapter 16 Screening Peptide Array Library for the Identification of Cancer Cell-Binding Peptides
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    Chapter 17 Next-generation sequencing of phage-displayed Peptide libraries.
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    Chapter 18 Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries
Attention for Chapter 12: Mapping protein-protein interactions with phage-displayed combinatorial Peptide libraries and alanine scanning.
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Chapter title
Mapping protein-protein interactions with phage-displayed combinatorial Peptide libraries and alanine scanning.
Chapter number 12
Book title
Peptide Libraries
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2020-4_12
Pubmed ID
Book ISBNs
978-1-4939-2019-8, 978-1-4939-2020-4
Authors

Malgorzata E Kokoszka, Brian K Kay, Malgorzata E. Kokoszka, Brian K. Kay, Kokoszka, Malgorzata E., Kay, Brian K.

Abstract

One avenue for inferring the function of a protein is to learn what proteins it may bind to in the cell. Among the various methodologies, one way for doing so is to affinity select peptide ligands from a phage-displayed combinatorial peptide library and then to examine if the proteins that carry such peptide sequences interact with the target protein in the cell. With the protocols described in this chapter, a laboratory with skills in microbiology, molecular biology, and protein biochemistry can readily identify peptides in the library that bind selectively, and with micromolar affinity, to a given target protein on the time scale of 2 months. To illustrate this approach, we use a library of bacteriophage M13 particles, which display 12-mer combinatorial peptides, to affinity select different peptide ligands for two different targets, the SH3 domain of the human Lyn protein tyrosine kinase and a segment of the yeast serine/threonine protein kinase Cbk1. The binding properties of the selected peptide ligands are then dissected by sequence alignment, Kunkel mutagenesis, and alanine scanning. Finally, the peptide ligands can be used to predict cellular interacting proteins and serve as the starting point for drug discovery.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 35%
Student > Master 5 22%
Student > Ph. D. Student 3 13%
Lecturer 1 4%
Student > Bachelor 1 4%
Other 0 0%
Unknown 5 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 22%
Agricultural and Biological Sciences 4 17%
Pharmacology, Toxicology and Pharmaceutical Science 2 9%
Neuroscience 2 9%
Veterinary Science and Veterinary Medicine 1 4%
Other 4 17%
Unknown 5 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 January 2015.
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#18,390,814
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Outputs from Methods in molecular biology
#7,875
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#255,698
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Outputs of similar age from Methods in molecular biology
#479
of 996 outputs
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