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Peptide Libraries

Overview of attention for book
Cover of 'Peptide Libraries'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Design, Synthesis, and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries.
  3. Altmetric Badge
    Chapter 2 Synthesis of Macrocyclic Organo-peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization.
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    Chapter 3 Synthesis and screening of one-bead-one-compound cyclic Peptide libraries.
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    Chapter 4 Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation
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    Chapter 5 Macrocyclic Templates for Library Synthesis of Peptido-Conjugates
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    Chapter 6 Synthesis and alkylation of aza-gly-pro building blocks of peptidomimetic libraries for developing prostaglandin f2α receptor modulators as therapeutics to inhibit preterm labor.
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    Chapter 7 A Fragment-Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases
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    Chapter 8 Peptide and Peptide Library Cyclization via Bromomethylbenzene Derivatives.
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    Chapter 9 High order unconditionally stable difference schemes for the Riesz space-fractional telegraph equation
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    Chapter 10 De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry
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    Chapter 11 Chemical Posttranslational Modification of Phage-Displayed Peptides
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    Chapter 12 Mapping protein-protein interactions with phage-displayed combinatorial Peptide libraries and alanine scanning.
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    Chapter 13 Identifying Reactive Peptides from Phage-Displayed Libraries
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    Chapter 14 The best and the brightest: exploiting tryptophan-sensitized tb(3+) luminescence to engineer lanthanide-binding tags.
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    Chapter 15 Synthesis and Cell-Based Screening of One-Bead-One-Compound Peptide Libraries
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    Chapter 16 Screening Peptide Array Library for the Identification of Cancer Cell-Binding Peptides
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    Chapter 17 Next-generation sequencing of phage-displayed Peptide libraries.
  19. Altmetric Badge
    Chapter 18 Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries
Attention for Chapter 17: Next-generation sequencing of phage-displayed Peptide libraries.
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Chapter title
Next-generation sequencing of phage-displayed Peptide libraries.
Chapter number 17
Book title
Peptide Libraries
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2020-4_17
Pubmed ID
Book ISBNs
978-1-4939-2019-8, 978-1-4939-2020-4
Authors

Wadim L Matochko, Ratmir Derda, Wadim L. Matochko, Matochko, Wadim L., Derda, Ratmir

Abstract

Genetically encoded peptide libraries enabled the discovery of ligands for clinically relevant targets and functional materials. Next-generation sequencing (NGS) of these libraries improved the selection of ligands by detecting low abundant clones and quantifying changes in copy numbers of clones without many rounds of selection. Although NGS platforms have been widely used in genome assembly, quantification of gene expression (RNA-seq), and metagenomic analyses, few examples in the literature describe sequencing phage libraries. This chapter aims to provide a detailed method for sequencing a Ph.D.-7 phage display library by Ion Torrent. The main techniques covered in this chapter include (1) preparation of a phage library for sequencing, (2) sequencing, and (3) analysis of the sequencing data by a custom Matlab script.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 47 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 23%
Researcher 9 19%
Student > Bachelor 8 17%
Student > Master 7 15%
Other 2 4%
Other 2 4%
Unknown 8 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 16 34%
Agricultural and Biological Sciences 7 15%
Chemistry 3 6%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Engineering 2 4%
Other 6 13%
Unknown 11 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2015.
All research outputs
#14,258,291
of 21,217,760 outputs
Outputs from Methods in molecular biology
#4,722
of 11,947 outputs
Outputs of similar age
#188,425
of 321,535 outputs
Outputs of similar age from Methods in molecular biology
#299
of 887 outputs
Altmetric has tracked 21,217,760 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,947 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
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We're also able to compare this research output to 887 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.