Mitosis

Chapter 1 Knock-in and knock-out: the use of reverse genetics in somatic cells to dissect mitotic pathways.

Chapter 2 Functional dissection of mitotic regulators through gene targeting in human somatic cells.

Chapter 3 RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression

Chapter 4 Production of mitotic regulators using an autoselection system for protein expression in budding yeast.

Chapter 5 Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis

Chapter 6 Isolation of protein complexes involved in mitosis and cytokinesis from Drosophila cultured cells.

Chapter 7 Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines

Chapter 8 Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos

Chapter 9 Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells

Chapter 10 Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells

Chapter 11 Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin Under the Native Promoter

Chapter 12 Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression

Chapter 13 Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts

Chapter 14 Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast

Chapter 15 The spindle checkpoint: assays for the analysis of spindle checkpoint arrest and recovery.

Chapter 16 Measuring proteolysis in mitosis.

Chapter 17 An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast

Chapter 18 In vitro assays for the anaphase-promoting complex/cyclosome (APC/C) in Xenopus egg extracts.

Chapter 19 Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation

Chapter 20 Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive (Pseudosubstrate) Inhibitors